Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-GAPDHS (bs-13282R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
- ￥1180 - 2800
- Bioss
- bs-13282R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13282R |
| 英文名称 | GAPDHS Rabbit pAb |
| 中文名称 | 精子3-磷酸甘油醛脱氢酶-2抗体 |
| 英文别名 | GAPDH2; GAPD2; GAPDH 2; GAPDH 2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase, testis specific; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; G3PT_HUMAN; HSD-35. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GAPDHS |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Homotetramer. Interacts with ARRB2; the interaction isdetected in the nucleus upon OR1D2 stimulation. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Testis specific. |
| 相似性 | Belongs to the glyceraldehyde-3-phosphatedehydrogenase family. |
| 功能 | May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a protein belonging to theglyceraldehyde-3-phosphate dehydrogenase family of enzymes thatplay an important role in carbohydrate metabolism. Like its somaticcell counterpart, this sperm-specific enzyme functions in anicotinamide adenine dinucleotide-dependent manner to removehydrogen and add phosphate to glyceraldehyde 3-phosphate to form1,3-diphosphoglycerate. During spermiogenesis, this enzyme may playan important role in regulating the switch between differentenergy-producing pathways, and it is required for sperm motilityand male fertility. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDHS Polyclonal Antibody, Unconjugated(bs-13282R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDHS Polyclonal Antibody, Unconjugated(bs-13282R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验该产品被引用文献
[IF={{ 6.63 }}] {Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17} {WB} {Mouse}
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[IF={{ 4.068 }}] {Ming Li. et al. ADAMTS12, a novel prognostic predictor, promotes cell proliferation, migration and invasion in head and neck squamous cell carcinoma. ORAL DIS. 2022 Oct;:} {WB} {Human}
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[IF={{ 1.918 }}] {Qingxin Fan. et al. Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes. Med Sci Monitor. 2021; 27: e928619-1–e928619-10} {WB} {}
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GAPDHS Rabbit pAb(bs-13282R)-50ul/100ul/200ul
￥1180 - 2800
