Blank control: A431.
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Primary Antibody (green line): Rabbit Anti-RIM2 antibody (bs-11357R)
_x000D_
Dilution: 3μg /10^6 cells;
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Isotype Control Antibody (orange line): Rabbit IgG .
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Secondary Antibody: Goat anti-rabbit IgG-AF647
_x000D_
Dilution: 3μg /test.
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Protocol
_x000D_
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-11357R
- 2026年10月13日
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- 技术资料
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11357R |
| 英文名称 | RIM2 Rabbit pAb |
| 中文名称 | 神经元突触膜胞外分泌调节蛋白2抗体 |
| 英文别名 | Non small cell lung cancer RimL3a protein; Rab3 interacting molecule 2; RAB3IP3; Regulating synaptic membrane exocytosis protein 2; Rims2; RIMS2_HUMAN. |
| 产品应用 | Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RIM2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 160 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Type Marker > Neuron marker > Synapse marker |
| 亚基 | Interacts with RAB3A and RAB3B that have been activated by GTP-binding. Interacts with RAB3C, RAB3D and RAB26. Interacts with BZRAP1/RIMBP1 and RIMBP2. Interacts with PPFIA3 and PPFIA4. Interacts via its zinc finger with the first C2 domain of UNC13A. Forms a complex consisting of UNC13A, RIMS2 and RAB3A. Heterodimer with PCLO. Part of a ternary complex involving PCLO and EPAC2 (By similarity). |
| 亚细胞定位 | Cell membrane; Peripheral membrane protein (By similarity). Cell junction, synapse (By similarity). Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein (By similarity). |
| 相似性 | Contains 2 C2 domains. Contains 1 FYVE-type zinc finger. Contains 1 PDZ (DHR) domain. Contains 1 RabBD (Rab-binding) domain._x000D_ |
| 功能 | RIM2 is a rab effector involved in exocytosis. It may act as scaffold protein. It is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP dependent manner. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Rab3, a neural/neuroendocrine-specific member of the Rab family, is involved in Ca2+-regulated exocytosis (1-2). Rab3 functions in an inhibitory capacity by controlling the recruitment of secretory vesicles into a releasable pool at the plasma membrane. Rim (rab3 interacting molecule), a putative effector protein for Rab3s, is composed of an amino-terminal zinc-finger motif and carboxy-terminal PDZ and C2 domains. Rim exists as two variants, Rim1 and Rim2, produced by alternative splicing (3). Rim1 is expressed near the active zone at the synapse, where it interacts in a GTP-dependent manner with Rab3, located on synaptic vesicles (4). Therefore, Rim serves as a Rab3-dependent regulator of synaptic-vesicle fusion by forming a GTP-dependent complex between synaptic plasma membranes and docked synaptic vesicles (5). Both Rim1 and Rim2 can bind to cAMP-GEFII, which is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-dependent exocytosis (3). Rim also localizes on the plasma membrane of INS-1E cells and pancreatic beta-cells. Rab3 binding domain of Rim enhances glucose-stimulated secretion in intact cells and Ca2+-stimulated exocytosis in permeabilized cells, suggesting that Rim may also play a regulatory role in insulin secretion (6). |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {3ug/Test} |
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RIMS2 | RIM2 Rabbit pAb(bs-11357R)-50ul/100ul/200ul
￥1180 - 2800
