Tissue/cell: Mouse testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NEK9 Polyclonal Antibody, Unconjugated(bs-9317R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-9317R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9317R |
| 英文名称 | NEK9 Rabbit pAb |
| 中文名称 | 丝氨酸/苏氨酸蛋白激酶NEK9抗体 |
| 英文别名 | Nek 8; NEK 9; Nek8; nek9; Nek-8; nek-9; NEK9_HUMAN; NERCC 1; Nercc 1 kinase; NERCC; NERCC1; Nercc1 kinase; Never in mitosis A related kinase 9; Never in mitosis A-related kinase 9; NIMA(never in mitosis gene a) related kinase 9; NIMA related kinase 8; NIMA related kinase 9; NIMA related kinase Nek 8; NIMA related kinase Nek 9; NIMA related kinase Nek8; NIMA related kinase Nek9; NimA related protein kinase 9; NIMA-related kinase 8; NimA-related protein kinase 9; Serine/threonine protein kinase Nek 9; Serine/threonine protein kinase Nek9; Serine/threonine-protein kinase Nek9. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NEK9 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 107 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Division > Spindle Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases Signal Transduction > Protein Phosphorylation > Tyrosine Kinases |
| 亚基 | Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex. |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 组织特异性 | Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts. |
| 翻译后修饰 | Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. [SIMILARITY] Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. [SIMILARITY] Contains 1 protein kinase domain. |
| 相似性 | Contains 6 RCC1 repeats. |
| 功能 | Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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NEK9 Rabbit pAb(bs-9317R)-50ul/100ul/200ul
￥1180 - 2800
