Sample:
_x000D_
K562(Human) Cell Lysate at 30 ug
_x000D_
Primary: Anti-Phospho-eNOS (Thr495) (Ser319) (bs-3731R) at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 133 kD
_x000D_
Observed band size: 133 kD
- ￥1180 - 2800
- Bioss
- bs-3731R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3731R |
| 英文名称 | phospho-eNOS (Thr495) Rabbit pAb |
| 中文名称 | 磷酸化一氧化氮合成酶3（内皮型）抗体 |
| 英文别名 | eNOS(phospho T494)mo, rat; eNOS(phospho T495)human; p-eNOS(phospho T495)human; eNOS(phospho-Thr495)human; cNOS; eNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3(endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Rabbit, Sheep, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human eNOS around the phosphorylation site of Thr495 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 133 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cardiovascular > Blood > Platelets Cardiovascular > Vasculature > Vasodilation > Nitric oxide associated Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Neuroscience > Neurotransmission > Nitric Oxide > NOS |
| 亚基 | Homodimer. Interacts with NOSIP and NOSTRIN. |
| 亚细胞定位 | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| 组织特异性 | Platelets, placenta, liver and kidney. |
| 翻译后修饰 | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. |
| 相似性 | Belongs to the NOS family. _x000D_ Contains 1 FAD-binding FR-type domain. _x000D_ Contains 1 flavodoxin-like domain. |
| 功能 | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets._x000D_
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2ug/Test} |
Sample:
_x000D_ Lane1: Liver (Mouse) Lysate at 30 ug
_x000D_ Lane2: Kidney (Mouse) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Phospho-eNOS (bs-3731R) at 1:300 dilution;
_x000D_ Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
_x000D_ Predicted band size : 133kD
_x000D_ Observed band size : 135kD
_x000D_ Lane1: Liver (Mouse) Lysate at 30 ug
_x000D_ Lane2: Kidney (Mouse) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Phospho-eNOS (bs-3731R) at 1:300 dilution;
_x000D_ Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
_x000D_ Predicted band size : 133kD
_x000D_ Observed band size : 135kD
Blank control:U937.
_x000D_ Primary Antibody (green line): Rabbit Anti-Phospho-eNOS (Thr495) antibody (bs-3731R)
_x000D_ Dilution: 2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-Phospho-eNOS (Thr495) antibody (bs-3731R)
_x000D_ Dilution: 2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
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文献和实验该产品被引用文献
[IF=6.656] Jie Chen. et al. Triterpenoid saponins from Ilex pubescens promote blood circulation in blood stasis syndrome by regulating sphingolipid metabolism and the PI3K/AKT/eNOS signaling pathway. PHYTOMEDICINE. 2022 Jun;:154242 IHC Rat
[IF=4.99] Papinska, A. M., et al. "Angiotensin‐(1‐7) administration benefits cardiac, renal, and progenitor cell function in db/db mice." British Journal of Pharmacology(2015). IHC ="Mouse"
[IF=4.8] Bruno Askiel. et al. A randomized blinded dose–response human biomarker study of remote ischemic conditioning. BRAIN CIRC. 2025 Jul;:10.4103/bc.bc_42_25 FC Human
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NOS3 | eNOS (p-T495) Rabbit pAb(bs-3731R)-50ul/100ul/200ul
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