Sample:
Heart (Mouse) Lysate at 40 ug
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-API5 (bs-1252R) at 1:300
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000 dilution
Predicted band size : 57 kD
Observed band size :57 kD
- ￥1180 - 2800
- Bioss
- bs-1252R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 详细信息
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1252R |
| 英文名称 | API5 Rabbit pAb |
| 中文名称 | 凋亡抑制因子5抗体 |
| 英文别名 | apoptosis inhibitor 5; apoptosis inhibitory protein 5; API5; AAC-11; AAC11; API5L1; AAC 11; Antiapoptosis clone 11 protein; API5 like 1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; Migration inducing protein MIG8; MIG 8; MIG8; Protein XAGL; API5_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, Horse, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human API5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 57 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Apoptosis > Receptors > Death receptors & ligands > IAPs Cancer > Invasion/microenvironment > Apoptosis > Death receptors & ligands > IAPs Cell Biology > Apoptosis > Intracellular > Survivin / IAPs |
| 亚基 | Monomer. Interacts with FGF2 and ACIN1. |
| 亚细胞定位 | Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic. Isoform 3: Cytoplasm. |
| 组织特异性 | Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. |
| 翻译后修饰 | Acetylation at Lys-251 impairs antiapoptotic function. |
| 相似性 | Belongs to the API5 family. |
| 功能 | Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. API5 expression is often upregulated in tumor cells, particularly in metastatic cells. It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription. Alternate Names: AAC 11; AAC11; Antiapoptosis clone 11 protein; API 5; API5; API5 like 1; API5L1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL.Function : Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (API5) Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (API5) Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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API5 Rabbit pAb(bs-1252R)-50ul/100ul/200ul
￥1180 - 2800
