DNTT | TDT Rabbit pAb(bs-2938R

Blank control (Black line):Molt4 (Black).
_x000D_ Primary Antibody (green line): Rabbit Anti-TdT antibody (bs-2938R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

P‌‌araformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TdT) Polyclonal Antibody, Unconjugated (bs-2938R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Molt-4 cells were fixed with 4% PFA for 10min at room temperature ,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TdT Antibody(bs-2938R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).