Blank control: A549.
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Primary Antibody (green line): Rabbit Anti-TRPC1 antibody (bs-10404R)
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Dilution: 3μg /10^6 cells;
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Isotype Control Antibody (orange line): Rabbit IgG .
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Secondary Antibody : Goat anti-rabbit IgG-PE
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Dilution: 1μg /test.
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Protocol
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The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-10404R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10404R |
| 英文名称 | TRPC1 Rabbit pAb |
| 中文名称 | 瞬时受体电位通道1抗体 |
| 英文别名 | TRPC1_HUMAN; Short transient receptor potential channel 1; TrpC1; Transient receptor protein 1; TRP-1; TRP 1; TRP 1 protein; TRP1; TRP1 protein; .HTRP 1; HTRP1; MGC133334; MGC133335; Mtrp1; Transient receptor potential cation channel subfamily C member 1; Transient receptor potential channel 1; Trp related protein 1; TRPC1; Trpc1 transient receptor potential cation channel subfamily C member 1. |
| 产品应用 | Flow-Cyt=3ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TRPC1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 87 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Hypertrophy Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Channels |
| 亚基 | Interacts with TRPC4AP (By similarity). Homotetramer and heterotetramer with TRPC4 and/or TRPC5. Interacts with TRPC3, TRPC4 and TRPC5. Interacts with ITPR3. Interacts with MX1 and RNF24. Interacts with FKBP4. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Seems to be ubiquitous. |
| 相似性 | Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily. |
| 功能 | Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {3ug/test} |
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[IF=5.656] Guo J et al. Transient Receptor Potential Canonical 5-Scramblase Signaling Complex Mediates Neuronal Phosphatidylserine Externalization and Apoptosis. Cells. 2020 Feb 26;9(3). IP human
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TRPC1 | TRP-1 Rabbit pAb(bs-10404R)-50ul/100ul/200ul
￥1180 - 2800
