- ￥1180 - 2800
- Bioss
- bs-6796R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6796R |
| 英文名称 | CIDEC Rabbit pAb |
| 中文名称 | 细胞死亡活化蛋白抗体 |
| 英文别名 | Cell Death Activator; Cell death activator CIDE-3; Cell Death Inducing DFFA Like Effector C; Cell death inducing DFFA like effector protein C; Cell death-inducing DFFA-like effector protein C; CIDE 3; CIDE3; CIDE C; CIDEC_HUMAN; Fat specific protein 27; Fat-specific protein FSP27 homolog; FLJ20871; FSP27. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CIDEC |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 27 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with CEBPB (By similarity). Interacts withCIDEA. |
| 亚细胞定位 | Nucleus (By similarity). Endoplasmicreticulum (By similarity). Lipid droplet. Note=Diffuses quickly onlipid droplet surface, but becomes trapped and clustered at lipiddroplet contact sites, thereby enabling its rapid enrichment atlipid droplet contact sites. |
| 组织特异性 | Expressed mainly in adipose tissue, smallintestine, heart, colon and stomach and, at lower levels, in brain,kidney and liver. |
| 翻译后修饰 | Ubiquitinated and targeted to proteasomal degradation,resulting in a short half-life. Protein stability depends ontriaclyglycerol synthesis, fatty acid availability and lipiddroplet formation (By similarity). |
| 相似性 | Contains 1 CIDE-N domain. |
| 功能 | May act as a CEBPB coactivator in white adipose tissueto control the expression of a subset of CEBPB downstream targetgenes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (Bysimilarity). Binds to lipid droplets and regulates theirenlargement, thereby restricting lipolysis and favoring storage. Atfocal contact sites between lipid droplets, promotes directionalnet neutral lipid transfer from the smaller to larger lipiddroplets. The transfer direction may be driven by the internalpressure difference between the contacting lipid droplet pair. Whenoverexpressed in preadipocytes, induces apoptosis or increases cellsusceptibility to apoptosis induced by serum deprivation or TGFBtreatment. As mature adipocytes, that express high CIDEC levels,are quite resistant to apoptotic stimuli, the physiologicalsignificance of its role in apoptosis is unclear. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010].Tissue specificity: Expressed mainly in small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CIDEC antibody (bs-6796R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CIDEC antibody (bs-6796R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CIDEC antibody (bs-6796R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CIDEC antibody (bs-6796R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with CIDEC polyclonal antibody, unconjugated (bs-6796R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 8.2 }}] {Yingjun Zhou. et al. Auricularia auricula-judae (Bull.) polysaccharides improve obesity in mice by regulating gut microbiota and TLR4/JNK signaling pathway. INT J BIOL MACROMOL. 2023 Oct;250:126172} {WB} {Mouse}
[IF={{ 6.656 }}] {Qing-song Xia. et al. Ban-xia-xie-xin-tang ameliorates hepatic steatosis by regulating Cidea and Cidec expression in HFD-fed mice. PHYTOMEDICINE. 2022 Oct;105:154351} {WB,IHC} {Mouse}
[IF={{ 2.742 }}] {Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7} {WB} {Mouse}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
技术资料暂无技术资料 索取技术资料
文献支持
CIDEC Rabbit pAb(bs-6796R)-50ul/100ul/200ul
￥1180 - 2800
