- ￥2980
- Bioss
- bs-2577R-HRP
- 2026年01月17日
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100ul
| 产品编号 | bs-2577R-HRP |
| 英文名称 | GNLY Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的颗粒溶素抗体 |
| 英文别名 | Granulysin; D2S69E; LAG2; 519; LAG 2; Lymphocyte activation gene 2; Lymphokine LAG 2; NKG 5; NKG5; Protein NKG5; T cell activation protein 519; T lymphocyte activation gene 519; TLA519. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GNLY/NKG5 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells Immunology > Innate Immunity > NK Cells |
| 亚细胞定位 | Secreted. Note=Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation. |
| 组织特异性 | Expressed in natural killer and T-cells. |
| 翻译后修饰 | A 9 kDa form is produced by proteolytic processing of a 15 kDa protein. |
| 相似性 | Contains 1 saposin B-type domain. |
| 功能 | Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GNLY (granulysin) is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. GNLY has antimicrobial activity against M.tuberculosis and other organisms including Gram-positive and Gram-negative bacteria, fungi, and parasites. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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