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- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5885R |
| 英文名称 | Fast Myosin Skeletal Heavy chain Rabbit pAb |
| 中文名称 | 肌球蛋白重链抗体 |
| 英文别名 | heavy chain Myosin; adult 1; MGC133384; Myh 1; Myh1; MYH1_HUMAN; Myosin heavy chain; Myosin heavy chain IIx/d; Myosin heavy chain skeletal muscle adult 1; Myosin light chain 1 alkali skeletal fast; Myosin-1; Myosin1; skeletal muscle; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1; MYH1_HUMAN; MYL1; Myosin-1; Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain IIx/d; MyHC-IIx/d; Myosin heavy chain, skeletal muscle, adult 1. |
| 产品应用 | ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Chicken, Dog, Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Myosin heavy chain 1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 223 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Motor Proteins > Myosin |
| 亚基 | Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). |
| 亚细胞定位 | Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. |
| 相似性 | Contains 1 IQ domain.
Contains 1 myosin head-like domain. |
| 功能 | Muscle contraction. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 7.014 }}] {Kong D et al. MiR-17 and miR-19 cooperatively promote skeletal muscle cell differentiation. Cell Mol Life Sci. 2019 Jun 18.} {ICC} {Human}
[IF={{ 5.1 }}] {Zongyun Du. et al. Fin Cells as a Promising Seed Cell Source for Sustainable Fish Meat Cultivation. FOODS. 2025 Jan;14(12):2075} {IF} {Fish}
[IF={{ 4.755 }}] {Zhang Lijiao. et al. Dysregulated myokines and signaling pathways in skeletal muscle dysfunction in a cigarette smoke–induced model of chronic obstructive pulmonary disease. FRONT PHYSIOL. 2022 Aug;0:1600} {IHC,WB} {Mouse}
[IF={{ 2.674 }}] {Tugeqin Bou. et al. Fast and slow myofiber-specific expression profiles are affected by noncoding RNAs in Mongolian horses. Comp Biochem Phys D. 2022 Mar;41:100942} {IHC} {Horses}
[IF={{ 2.573 }}] {Bao T et al. The distinct transcriptomes of fast-twitch and slow-twitch muscles in Mongolian horses. Comp Biochem Physiol Part D Genomics Proteomics. 2019 Dec 11;33:100649.} {IHC} {Horse}
Protein purification: skeletal muscle myosin
Overview SKELETAL MUSCLE MYOSIN A two-headed hexomeric actin-activated ATPase consisting of two heavy chains of ~200 kDa and four light chains of ~20kDa. The tail portion
Determination of Muscle Fiber Type in Rodents
consist of muscle fibers that can differ in both composition and functional characteristics. These three types of muscle fibers, broadly categorized as slow, fast IIa, and fast IIb muscle fibers, express characteristic myosin heavy chain proteins
by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse IgG1 heavy chain do stain rat IgG2a and IgG2b . Procedure: First stain HRP Developing solution Second stain AP
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