Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-Utrophin antibody (bs-11394R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-11394R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11394R |
| 英文名称 | Utrophin Rabbit pAb |
| 中文名称 | 肌营养不良蛋白相关蛋白1抗体 |
| 英文别名 | DMDL; DRP 1; DRP; DRP-1; DRP1; Dystrophin like protein; Dystrophin related protein 1; Dystrophin related protein; Dystrophin-related protein 1; FLJ23678; UTRN; UTRO_HUMAN; Utrophin(homologous to dystrophin); Utrophin |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Hela (Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Utrophin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 394 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Type Marker > Neuron marker > Synapse marker Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Binding Proteins |
| 亚基 | Interacts with the syntrophins SNTA1; SNTB1 and SNTB2. Interacts with SYNM. Interacts (via its WWW and ZZ domains) with DAG1 (via the PPXY motif of betaDAG1); the interaction is inhibited by the tyrosine phosphorylation of the PPXY motif of DAG1. |
| 亚细胞定位 | Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Neuromuscular junction. |
| 组织特异性 | Muscle. |
| 相似性 | Contains 2 CH (calponin-homology) domains. Contains 20 spectrin repeats. Contains 1 WW domain. Contains 1 ZZ-type zinc finger. |
| 功能 | May play a role in anchoring the cytoskeleton to the plasma membrane. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Dystrophin and utrophin are related structural, Actin-binding proteins that are involved in anchoring the cytoskeleton to the plasma membrane. Dystrophin is the protein product of the Duchenne/Becker muscular dystrophy gene. Dystrophin expression is found in muscle and brain tissues, where it is localized to the inner surface of the plasma membrane. It has been speculated that alternative splicing of the carboxy terminus allows dystrophin to interact with a variety of proteins. Research has shown that the loss of dystrophin-associated proteins in Duchenne afflicted muscle is due to the absence of dystrophin rather than to muscle degradation and that the lack of dystrophin results in the loss of linkage between the cytoskeleton and the extracellular matrix. Evidence suggests that the upregulation of utrophin can reduce the dystrophic pathology. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
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Utrophin Rabbit pAb(bs-11394R)-50ul/100ul/200ul
￥1180 - 2800
