Blank control（black line）:Hela.
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Primary Antibody (green line): Rabbit Anti-MtTF1/TFAM antibody (bs-8520R)
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Dilution:1ug/Test;
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Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
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Dilution: 0.5ug/Test.
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Isotype control（orange line）: Normal Rabbit IgG
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Protocol
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The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-8520R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8520R |
| 英文名称 | MtTF1/TFAM Rabbit pAb |
| 中文名称 | 线粒体转录因子1抗体 |
| 英文别名 | Mitochondrial transcription factor 1; mitochondrial transcription factor A; MtTF1; mtTFA; TCF 6; TCF-6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; TFAM_HUMAN; Transcription factor 6; Transcription factor 6 like 2(mitochondrial transcription factor); Transcription factor 6 like 2; Transcription factor 6-like 2; Transcription factor A, mitochondrial; Transcription factor A, mitochondrial precursor. |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Hela (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MtTF1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Cardiogenesis > Transcription factors/regulators Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Metabolism > Pathways and Processes > Metabolic signaling pathways > Nucleotide metabolism > Molecular processes > Mitochondrial transcription Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial Biogenesis Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Signal Transduction > Metabolism > Mitochondrial Tags & Cell Markers > Subcellular Markers > Organelles > Mitochondria |
| 亚细胞定位 | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid. |
| 相似性 | Contains 2 HMG box DNA-binding domains. |
| 功能 | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. Target DNA. Interacts with TFB1M and TFB2M. Associates with mitochondrial DNA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
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文献和实验该产品被引用文献
[IF=4.966] Guangning Kou. et al. Nobiletin activates thermogenesis of brown and white adipose tissue in high‐fat diet‐fed C57BL/6 mice by shaping the gut microbiota. Faseb J. 2021 Feb;35(2):e21267 WB Mouse
[IF=3.974] Ning X et al.Ambient PM2. 5 causes lung injuries and coupled energy metabolic disorder. (2018) Ecotoxicol. Environ. Saf. 170 WB Mouse
[IF=3.95] Yan, Wei, et al. "Acute nitrogen dioxide inhalation induces mitochondrial dysfunction in rat brain." Environmental Research 138 (2015): 416-424. WB ="Rat"
[IF=3.258] Liu H et al. Cybrid Model Supports Mitochondrial Genetic Effect on Pig Litter SizeFront Genet.2020 Dec 15;11:579382. WB pig
[IF=3.2] Savran Ozgur Yildirim. et al. MitoTEMPO modulates trigeminal activation and mitochondrial biogenesis in a nitroglycerin-induced migraine model. MOL BIOL REP. 2026 Dec;53(1):742 WB Rat
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TFAM | mtTFA/mtTF1 Rabbit pAb(bs-8520R)-50ul/100ul/200ul
￥1180 - 2800
