- ￥1180 - 2800
- Bioss
- bs-7552R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7552R |
| 英文名称 | Biglycan Rabbit pAb |
| 中文名称 | 骨/软骨蛋白多糖1抗体 |
| 英文别名 | BGN; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Biglycan |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 37 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Other ECM Proteins Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone Stem Cells > Mesenchymal Stem Cells > Osteogenesis |
| 亚基 | Homodimer. Forms a ternary complex with MFAP2 and ELN |
| 亚细胞定位 | Secreted, extracellular space, extracellular. |
| 组织特异性 | Found in several connective tissues, especially in articular cartilages. |
| 翻译后修饰 | The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity). |
| 相似性 | Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats. |
| 功能 | May be involved in collagen fiber assembly (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | May be involved in collagen fiber assembly. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (bs-7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
_x000D_ Colon (Rat) Lysate at 40 ug
_x000D_ Embryo (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Biglycan (bs-7552R) at 1/300 dilution
_x000D_ Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
_x000D_ Predicted band size: 37 kD
_x000D_ Observed band size: 52 kD
_x000D_ _x000D_
_x000D_ Colon (Rat) Lysate at 40 ug
_x000D_ Embryo (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Biglycan (bs-7552R) at 1/300 dilution
_x000D_ Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
_x000D_ Predicted band size: 37 kD
_x000D_ Observed band size: 52 kD
_x000D_ _x000D_
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
Sample: Bone (mouse) Lysate at 40 ug
_x000D_ Primary: Anti- Biglycan (bs-7552R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 37kD
_x000D_ Observed band size: 52 kD
_x000D_
_x000D_ Primary: Anti- Biglycan (bs-7552R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 37kD
_x000D_ Observed band size: 52 kD
_x000D_
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BGN | Biglycan Rabbit pAb(bs-7552R)-50ul/100ul/200ul
￥1180 - 2800
