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Biglycan Rabbit pAb(bs-7552R)-

50ul/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-7552R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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    • 规格

      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-7552R
    英文名称Biglycan Rabbit pAb
    中文名称骨/软骨蛋白多糖1抗体
    英文别名BGN; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Biglycan
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量37 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Other ECM Proteins

    Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone

    Stem Cells > Mesenchymal Stem Cells > Osteogenesis

    亚基Homodimer. Forms a ternary complex with MFAP2 and ELN
    亚细胞定位Secreted, extracellular space, extracellular.
    组织特异性Found in several connective tissues, especially in articular cartilages.
    翻译后修饰The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity).
    相似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
    功能May be involved in collagen fiber assembly (By similarity).
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料May be involved in collagen fiber assembly.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (bs-7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    Sample:
    Colon (Rat) Lysate at 40 ug
    Embryo (Mouse) Lysate at 40 ug
    Primary: Anti-Biglycan (bs-7552R) at 1/300 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
    Predicted band size: 37 kD
    Observed band size: 52 kD
    产品细节图片3
    Tissue/cell: human lung carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片4
    Sample: Bone (mouse) Lysate at 40 ug
    Primary: Anti- Biglycan (bs-7552R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37kD
    Observed band size: 52 kD

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    图标文献和实验
    该产品被引用文献

    [IF={{ 9.8 }}] {Xiaotong Wang. et al. Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets. SCI TOTAL ENVIRON. 2024 Jan;908:168303} {IF} {Pig}

    [IF={{ 8.554 }}] {Jin Wang. et al. TLR4 aggravates microglial pyroptosis by promoting DDX3X-mediated NLRP3 inflammasome activation via JAK2/STAT1 pathway after spinal cord injury. CLIN TRANSL MED. 2022 Jun;12(6):e894} {IP,WB} {Mouse}

    [IF={{ 5.23 }}] {Han, Chao, et al. "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." Scientific Reports 6 (2016): 35102.} {WB} {="Rabbit"}

    [IF={{ 2.36 }}] {Simões et al. Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women: a pilot study. (2017) J.Ovarian.Res. 10:54} {IHC} {Human}

    [IF={{ 1.72 }}] {Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014).} {IHC} {="Rabbit"}

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    Biglycan Rabbit pAb(bs-7552R)-50ul/100ul/200ul
    ¥1180 - 2800