NF-H Rabbit pAb(bs-10680R)-50ul/100ul/200ul
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NF-H Rabbit pAb(bs-10680R)-50u

l/100ul/200ul
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  • ￥1180 - 2800
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  • bs-10680R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-10680R
    英文名称NF-H Rabbit pAb
    中文名称高分子量神经丝蛋白抗体
    英文别名NFH_HUMAN; Neurofilament heavy polypeptide; NEFH; KIAA0845; NFH; NF H; NF200; NF-200; Neurofilament 200; 200 kDa neurofilament protein; Neurofilament triplet H protein; Neurofilament H; Neurofilament heavy polypeptide 200kD;
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Mouse, Dog, Pig, Cow, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human NF-H
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量118 kDa
    实际分子量200 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Intermediate Filaments

    Neuroscience > Cell Type Marker > Neuron marker > Axon marker

    Neuroscience > Neurology process > Neurodegenerative disease

    Neuroscience > Neurology process > Neurodegenerative disease > Alzheimer's disease

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class IV > Neurofilaments

    翻译后修饰There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    相似性Belongs to the intermediate filament family.
    功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia.

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1:ug/Test}

     

    NF-H Rabbit pAb(bs-10680R)-50u
    Blank control (Black line): Molt4 (Black).
    Primary Antibody (green line):Rabbit Anti-NF-H antibody (bs-10680R)
    Dilution:1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    NF-H Rabbit pAb(bs-10680R)-50u
    Tissue/cell:SH-SY5Y cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (NF-H) polyclonal Antibody, Unconjugated (bs-10680R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    NF-H Rabbit pAb(bs-10680R)-50u
    Tissue/cell: rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-NF-H Polyclonal Antibody, Unconjugated(bs-10680R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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    图标文献和实验
    该产品被引用文献

    [IF={{ 3.37 }}] {Liu, Yi, et al. "Conserved Dopamine Neurotrophic Factor-Transduced Mesenchymal Stem Cells Promote Axon Regeneration and Functional Recovery of Injured Sciatic Nerve." PLOS ONE 9.10 (2014): e110993.} {IHC} {="Rat"}

    [IF={{ 3.3 }}] {Juan Zhang. et al.Up-regulation of miR-10a-5p expression inhibits the proliferation and differentiation of neural stem cells by targeting Chl1.Acta Biochimica et Biophysica Sinica.2024 Jun 5;56(10):1483-1497.} {ICC} {Mouse}

    [IF={{ 3.26 }}] {Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).} {IHC} {="Chicken"}

    [IF={{ 2.9 }}] {Jinjie Ma. et al.The potential role of SCF combined with DPCs in facial nerve repair.JOURNAL OF MOLECULAR HISTOLOGY.2025 Jan 7;56(1):67.} {IF} {Rabbit}

    [IF={{ 2.276 }}] {Zhang Heng. et al. Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro. Biomed Res Int. 2021;2021:7239783} {WB,IF,ICC} {}

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    NF-H Rabbit pAb(bs-10680R)-50ul/100ul/200ul
    ￥1180 - 2800