PIK3R2 Rabbit pAb(bs-4160R)-50ul/100ul/200ul
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PIK3R2 Rabbit pAb(bs-4160R)-50

ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-4160R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-4160R
    英文名称PIK3R2 Rabbit pAb
    中文名称磷脂酰肌醇激酶p85β抗体
    英文别名P85B_HUMAN; Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta(PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta); PI 3 Kinase p85 beta; PI 3-Kinase p85β; PI 3-Kinase p85-β; PI 3-Kinase p85 β; PI3 kinase p85 beta subunit; phosphoinositide 3 kinase regulatory subunit 2(beta); p85 beta; p85-BETA; P85B; PI3 Kinase p85 beta;
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Cow)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PI 3 Kinase p85 beta
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量82 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Immunology > Innate Immunity > TLR Signaling

    Signal Transduction > Signaling Pathway > Lipid Signaling > Lipid Kinases

    亚基Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated). Interacts with NYAP1, NYAP2 and MYO16.
    翻译后修饰Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ.
    相似性Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
    功能Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    PIK3R2 Rabbit pAb(bs-4160R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P85B) Polyclonal Antibody, Unconjugated (bs-4160R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    PIK3R2 Rabbit pAb(bs-4160R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (PIK3R2) Polyclonal/MonoclonalAntibody, Unconjugated (bs-4160R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
    PIK3R2 Rabbit pAb(bs-4160R)-50
    Sample: Colon carcinoma (Human) Lysate at 40 ug
    Primary: Anti- PI 3 Kinase p85 beta (bs-4160R) at 1/300 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
    Predicted band size: 82 kD
    Observed band size: 85 kD
    PIK3R2 Rabbit pAb(bs-4160R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (PIK3R2) Polyclonal/MonoclonalAntibody, Unconjugated (bs-4160R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
    PIK3R2 Rabbit pAb(bs-4160R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIK3R2) Polyclonal Antibody, Unconjugated (bs-4160R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    PIK3R2 Rabbit pAb(bs-4160R)-50
    Sample:
    Lane 1: Mouse Muscle tissue lysates
    Lane 2: Rat Muscle tissue lysates
    Primary: Anti-PIK3R2 (bs-4160R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 82 kDa
    Observed band size: 90 kDa

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    该产品被引用文献

    [IF={{ 7.8 }}] {Hongbo Ai. et al. Osteoclast-derived apoptotic bodies accelerate the pathological progression of osteoarthritis via disturbing subchondral bone remodeling. J ORTHOP TRANSL. 2025 Mar;51:108} {WB} {Mouse}

    [IF={{ 3.895 }}] {Yin H et al. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins (Basel). 2020 Jan 29;12(2).} {WB} {Chicken}

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    PIK3R2 Rabbit pAb(bs-4160R)-50ul/100ul/200ul
    ￥1180 - 2800