PRKCE | PKC epsilon Rabbit pAb(bs-2329R)-50ul/100ul/200ul产品图
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PRKCE | PKC epsilon Rabbit pAb

(bs-2329R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-2329R
    英文名称PKC epsilon Rabbit pAb
    中文名称蛋白激酶C抗体
    英文别名Protein kinase C epsilon type; MGC125656; MGC125657; nPKC epsilon; PKCE; Pkcea; PRKCE; Protein kinase C epsilon; PKC epsilon; KPCE_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PKCE
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量81 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > PKC

    亚基Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    亚细胞定位Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cytoplasm, perinuclear region. Nucleus. Note=Translocated to plasma membrane in epithelial cells stimulated by HGF. Associated with the Golgi at the perinuclear site in pre-passage fibroblasts. In passaging cells, translocated to the cell periphery. Translocated to the nucleus in PMA-treated cells.
    翻译后修饰hosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. Phosphorylation in the hinge domain at Ser-350 by MAPK11 or MAPK14, Ser-346 by GSK3B and Ser-368 by autophosphorylation is required for interaction with YWHAB.
    相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
    功能Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: rat testis tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    Tissue/cell: rat testis tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片3
    Tissue/cell: human kidney tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片4
    Sample:
    _x000D_ Lane 1: Mouse Cerebrum tissue lysates
    _x000D_ Lane 2: Mouse Cerebellum tissue lysates
    _x000D_ Lane 3: Rat Cerebrum tissue lysates
    _x000D_ Primary: Anti-PKC epsilon (bs-2329R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 81 kDa
    _x000D_ Observed band size: 100 kDa

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    图标文献和实验
    该产品被引用文献

    [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB Human

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    PRKCE | PKC epsilon Rabbit pAb(bs-2329R)-50ul/100ul/200ul
    ￥1180 - 2800