Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NPY2R Polyclonal Antibody, Unconjugated(bs-0937R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-0937R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0937R |
| 英文名称 | NPY2R Rabbit pAb |
| 中文名称 | 神经肽Y受体2抗体 |
| 英文别名 | Neuropeptide Y Receptor Type 2; Neuropeptide Y receptor Y2; NPY-Y2 receptor; NPY2R_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NPY2R |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Angiogenesis > Angiogenic Factors Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Atherosclerosis > Lipoprotein metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipoprotein metabolism Metabolism > Types of disease > Cancer Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Neuroscience > Neurology process > Circadian Rhythm > Receptors Neuroscience > Neurotransmission > Receptors / Channels > GPCR > More GPCR |
| 亚细胞定位 | Cell membrane. |
| 组织特异性 | High levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu-31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Neuropeptide Y (NPY), a peptide widely expressed in the brain, acts through the protein G-coupled receptors Y1, Y2 and Y5. This peptide modulates many important functions such as the control of energy balance and anxiety. Its involvement in brain development has been less investigated. Neuropeptide Y (NPY) receptors are members of a G protein coupled receptor superfamily and they mediate a variety of physiological responses including feeding and vasoconstriction. The endogenous ligands of NPY receptors are a family of structurally related peptides, including NPY, peptide YY (PYY), and pancreatic polypeptide (PP). Increased activity of NPY and its receptors, Y1R, Y2R, and Y5R has been found in the brain in many forms of experimental obesity. Furthermore, several association studies have supported the association between NPY2R and obesity. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample: A549 Cell Lysate at 30 ug
Primary: Anti- NPY2R (bs-0937R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Primary: Anti- NPY2R (bs-0937R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-NPY2R (bs-0937R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 45 kDa
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-NPY2R (bs-0937R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 45 kDa
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= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
31600篇！CUSABIO 7月文献引用再破记录，200篇新文献为科研助力
期刊：Science Bulletin CUSABIO助力产品： Human neuropeptide Y (NPY) ELISA kit; CSB-E08168h 研究亮点 本研究揭示三叉神经痛中受损TG神经元上调并释放NPY，通过Y2R激活邻近痛觉神经元，同时招募并活化TG巨噬细胞，后者释放TNF-α增强神经元兴奋性，形成自我放大的神经-免疫正反馈环路，维持局部神经炎症和机械性痛觉过敏。靶向Y2R（BIIE0246）、基因敲除Npy2r或巨噬细胞清除均可显著逆转已建立的疼痛行为。临床数据显示TN患者
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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NPY2R Rabbit pAb(bs-0937R)-50ul/100ul/200ul
￥1180 - 2800
