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ZNF307/ZKSCAN3 Rabbit pAb(bs-1

940R)-50ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-1940R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1940R
    英文名称ZNF307/ZKSCAN3 Rabbit pAb
    中文名称锌指蛋白307抗体
    英文别名zinc finger with KRAB and SCAN domains 4; Zinc finger protein 307; P373c6.1; ZKSCAN4; FLJ32136; P1P373C6; p373c6.1(novel C2H2 type zinc finger protein); p373c6.1; Zinc finger protein 307; Zinc finger with KRAB and SCAN domains 4; ZKSCAN4; ZKSC4_HUMAN; dJ874C20.1; mszf35; SCAN-KRAB-zinc finger protein; Skz1; Zf47; Zfp-47; ZFP306; Zfp307; Zfp47; Zinc finger and SCAN domain containing protein 13; Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 306, isoform CRA_a; Zinc finger protein 307; Zinc finger protein 309; Zinc finger protein 47 homolog; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein zfp47; Zinc finger with KRAB and SCAN domains 3; ZKSC3_HUMAN; ZKSCAN3; ZNF309; ZSCAN13; ZSCAN35.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Dog, Cow, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human ZNF307
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量62 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚细胞定位Nucleus.
    组织特异性Expressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.
    相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
    Contains 1 SCAN box domain.
    功能May be involved in the transcriptional activation of MDM2 and EP300 genes.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料ZNF307 contains 1 SCAN box domain, 1 KRAB domain and 7 C2H2-type zinc fingers. It belongs to the Krueppel C2H2-type zinc-finger protein family and may be involved in transcriptional regulation. [SUBCELLULAR LOCATION] Nucleus. Expressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{2ug/Test}

     

    产品细节图片1
    Tissue/cell: mouse kidney tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    Tissue/cell: mouse fetal liver; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片3
    Tissue/cell: mouse kidney tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片4
    Tissue/cell: human kidney tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片5
    Sample:
    K562(Human) Cell Lysate at 40 ug
    Primary: Anti-ZNF307/ZKSCAN3 (bs-1940R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 62 kD
    Observed band size: 62 kD
    产品细节图片6
    SH-SY5Y cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ZNF307/ZKSCAN3) polyclonal Antibody, Unconjugated (bs-1940R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片7
    Blank control（black line）:SH-SY5Y.
    Primary Antibody (green line): Rabbit Anti-ZNF307/ZKSCAN3 antibody (bs-1940R)
    Dilution:2ug/Test;
    Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
    Dilution: 0.5ug/Test.
    Isotype control（orange line）: Normal Rabbit IgG
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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