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AACT Rabbit pAb(bs-0094R)-50ul

/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-0094R
更新日期2025年10月16日

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应用范围：
产品信息以Bioss网站为准
规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-0094R
英文名称	AACT Rabbit pAb
中文名称	α-1抗胰糜蛋白酶抗体
英文别名	AACT_HUMAN; SERPINA3; alpha-1-antichymotrypsin; ACT; Cell growth-inhibiting gene 24/25 protein; Serpin A3; GIG24; GIG25; serpin family A member 3;
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Mouse, Rat (Human)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human AACT
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	45 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cardiovascular > Blood > Serum Proteins Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases Neuroscience > Neurology process > Neurodegenerative disease > Alzheimer's disease > Proteases
亚基	Interacts with DNAJC1.
亚细胞定位	Secreted.
组织特异性	Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
相似性	Belongs to the serpin family.
功能	Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	Alpha 1-Antichymotrypsin, a member of the serine proteinase inhibitor (serpin) family, inhibits neutrophil proteinase cathepsin G and mast cell chymases, and protects the lower respiratory tract from damage by proteolytic enzymes. It contains a reactive centre loop, which interacts with cognate proteinases, resulting in loop cleavage and a major conformational change. Recently, alpha 1-antichymotrypsin has been identified as a major constituent of the neurofibrillary plaques associated with Alzheimers disease, and in vitro studies have shown that it enhances the rate of amyloid-fibril formation. These observations and recent genetic evidence suggest that alpha 1-antichymotrypsin is important in the pathogenesis of Alzheimers disease.

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}

Tissue/cell: Rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Alpha-1-Antichymotrypsin Polyclonal Antibody, Unconjugated (bs-0094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: Mouse liver tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Alpha-1-Antichymotrypsin Polyclonal Antibody, Unconjugated (bs-0094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Alpha-1-Antichymotrypsin (bs-0094R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD

Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Primary: Anti-Alpha-1-Antichymotrypsin (bs-0094R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa

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文献和实验

该产品被引用文献

[IF={{ 4.2 }}] {Jin Rao. et al. Integration of Microarray Data and Single-Cell Sequencing Analysis to Explore Key Genes Associated with Macrophage Infiltration in Heart Failure. J INFLAMM RES. 2024 Dec 19} {WB} {Mouse}

[IF={{ 3.3 }}] {Yan Zhang. et al. Proteomic analysis of multiple organ dysfunction induced by rhabdomyolysis. J PROTEOMICS. 2024 Apr;298:105138} {IHC,WB} {Rat}

[IF={{ 3.13 }}] {Wang, Robert YL, et al. "Proteome Demonstration of Alpha-1-Acid Glycoprotein and Alpha-1-Antichymotrypsin." Pediatric Infectious Disease Journal (2014).} {WB} {="Human"}

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