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- bs-8539R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8539R |
| 英文名称 | Luciferase Rabbit pAb |
| 中文名称 | 萤火虫荧光素酶抗体 |
| 英文别名 | Firefly Luciferase; ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase. |
| 产品应用 | ICC/IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Firefly) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from Firefly Luciferase |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 61 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > Luciferase |
| 亚细胞定位 | Peroxisome. |
| 相似性 | Belongs to the ATP-dependent AMP-binding enzyme family. |
| 功能 | Produces green light with a wavelength of 562 nm. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 6.814 }}] {Clara Escudero-Duch. et al. Remote control of transgene expression using noninvasive near-infrared irradiation. J PHOTOCH PHOTOBIO B. 2023 May;242:112697} {IHC} {Mouse}
[IF={{ 16.6 }}] {Fu Xinhua. et al. Key homeobox transcription factors regulate the development of the firefly’s adult light organ and bioluminescence. NAT COMMUN. 2024 Mar;15(1):1-18} {WB} {Aquatica leii}
[IF={{ 11.7 }}] {Junju Chen. et al. Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma. Cell Reports Medicine. 2024 Oct 30} {WB} {Human}
Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists
Firefly luciferase has been widely used as a model substrate to study folding (1 –3 ) and renaturation (4 –6 ) of protein because of its rapid and sensitive bloluminescenct activity. Although normally localized in peroxisomes, luciferase
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
are needed to reveal the important role of the members of the Wnt signaling pathway in the RA pathogenesis and treatment. Rspo 1 promotes osteoblast differentiation via Wnt signaling pathway. 作者：Sharma AR, Choi BS, Park JM, Lee DH, Lee JE, Kim HS
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