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- bs-8539R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8539R |
| 英文名称 | Luciferase Rabbit pAb |
| 中文名称 | 萤火虫荧光素酶抗体 |
| 英文别名 | Firefly Luciferase; ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase. |
| 产品应用 | ICC/IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Firefly) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from Firefly Luciferase |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 61 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > Luciferase |
| 亚细胞定位 | Peroxisome. |
| 相似性 | Belongs to the ATP-dependent AMP-binding enzyme family. |
| 功能 | Produces green light with a wavelength of 562 nm. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 6.814 }}] {Clara Escudero-Duch. et al. Remote control of transgene expression using noninvasive near-infrared irradiation. J PHOTOCH PHOTOBIO B. 2023 May;242:112697} {IHC} {Mouse}
[IF={{ 16.6 }}] {Fu Xinhua. et al. Key homeobox transcription factors regulate the development of the firefly’s adult light organ and bioluminescence. NAT COMMUN. 2024 Mar;15(1):1-18} {WB} {Aquatica leii}
[IF={{ 11.7 }}] {Junju Chen. et al. Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma. Cell Reports Medicine. 2024 Oct 30} {WB} {Human}
Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists
Firefly luciferase has been widely used as a model substrate to study folding (1 –3 ) and renaturation (4 –6 ) of protein because of its rapid and sensitive bloluminescenct activity. Although normally localized in peroxisomes, luciferase
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
(e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
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