Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hes5 Polyclonal Antibody, Unconjugated(bs-3767R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-3767R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3767R |
| 英文名称 | HES5 Rabbit pAb |
| 中文名称 | 发状分裂相关增强子-5抗体 |
| 英文别名 | BHLHB38; Transcription factor HES-5; Class B basic helix-loop-helix protein 38(bHLHb38); Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876; HES5_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Hes5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 18 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Neuroscience > Neurology process > Notch Pathway Stem Cells > Neural Stem Cells > Intracellular Stem Cells > Signaling Pathways > Notch > Nuclear |
| 亚基 | Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in fetal heart and brain tumors. |
| 相似性 | Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain. |
| 功能 | Transcriptional repressor of genes that require a bHLH protein for their transcription |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Kidney (Rat) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (bs-3767R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 20 kD
Kidney (Rat) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (bs-3767R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HES5) Polyclonal Antibody, Unconjugated (bs-3767R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HES5) Polyclonal Antibody, Unconjugated (bs-3767R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 6.119 }}] {Liu D et al. PNO1, which is negatively regulated by miR-340-5p, promotes lung adenocarcinoma progression through Notch signaling pathway. Oncogenesis. 2020 Jun 1;9(5):58.} {WB} {Human}
[IF={{ 3.1 }}] {Yang Chan Mo. et al. Comprehensive molecular profiling of combined hepatocellular carcinoma and cholangiocarcinoma reveals distinct Notch signaling subgroups with prognostic significance. VIRCHOWS ARCH. 2025 Jul;:1-13} {IHC} {Human}
[IF={{ 2.96 }}] {Zhang, Haihua, et al. "Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway." Biochimie (2016).} {WB} {="Other Species"}
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HES5 Rabbit pAb(bs-3767R)-50ul/100ul/200ul
￥1180 - 2800
