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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1910R |
| 英文名称 | TGF beta Receptor III Rabbit pAb |
| 中文名称 | 转移生长因子β受体3抗体 |
| 英文别名 | beta glycan; TGF beta R3; betaglycan; betaglycan proteoglycan; BGCAN; TGF beta receptor type 3; TGF-beta receptor type 3; TGF beta receptor type III; TGF-beta receptor type III; TGFB R3; TGFBR 3; TGFBR3; TGFR 3; TGFR-3; TGFR3; Transforming Growth Factor beta Receptor III; Transforming growth factor beta receptor III betaglycan 300kDa; TGF-βRⅢ; TGFβRⅢ; TGFβ RIII; TGF β RIII; TGF-β RIII; TGFβ-RIII; Transforming growth factor beta receptor type 3. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human TGFBR3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 94 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with TCTEX1D4. |
| 亚细胞定位 | Secreted. Secreted > extracellular space. Cell membrane. Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix. |
| 翻译后修饰 | Extensively modified by glycosaminoglycan groups (GAG). |
| 相似性 | Contains 1 ZP domain. |
| 功能 | Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.[provided by RefSeq, Sep 2010]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
K562 Cell (Human) Lysate at 40 ug
HepG2 Cell (Human) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Primary: Anti- TGF beta Receptor III (bs-1910R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kD
Observed band size: 110 kD
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human A431 cell lysates
Primary: Anti-TGF beta Receptor III (bs-1910R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 130 kDa
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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
） osteocalcin HOXA10 N-MYC NDRG-1 ck20 mmp-7 DAT1 （Dopamine Transporter gene) β-globin IL-6 TGF-β IFN-γ FasL RNA helicase cyp2e1(NM_000773) p53(NM_000546) Fucosyltransferase III/IV/V/ VII (α-1,3岩藻糖基转移酶III/IV/V/ VII ) c-fos c-jun c-myc IL-24
, Yoon JK, Song DK, Nam JS, Lee SS. R-spondin (Rspo)s proteins are a new group of Wnt/beta-catenin signaling agonists. These signaling molecules are known to be involved in the developmental stages of skeletal system. Recent studies in various murine
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