Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-Glutamine synthetase Polyclonal Antibody, Unconjugated(bs-4143R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- ￥1180 - 2800
- Bioss
- bs-4143R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4143R |
| 英文名称 | Glutamine synthetase Rabbit pAb |
| 中文名称 | 谷氨酰胺合成酶/谷氨酸氨连接酶抗体 |
| 英文别名 | GLUL; cell proliferation-inducing protein 59; GLNA; GLNA_HUMAN; GLNS; Glutamate ammonia ligase; Glutamate decarboxylase; Glutamate--ammonia ligase; glutamine synthase; GS; PIG 43; PIG 59; PIG43; PIG59; Proliferation inducing protein 43. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Glutamine synthetase |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 42 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurology process > Neurodegenerative disease > Alzheimer's disease Neuroscience > Neurotransmitter > Amino Acids > Glutamate |
| 亚细胞定位 | Cytoplasm. Mitochondrion. |
| 相似性 | Belongs to the glutamine synthetase family. |
| 功能 | This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 42 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
Sample: Raji Cell (Human) Lysate at 40 ug
_x000D_ Primary: Anti- Glutamine synthetase (bs-4143R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 42 kD
_x000D_ Observed band size: 42 kD
_x000D_
_x000D_ Primary: Anti- Glutamine synthetase (bs-4143R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 42 kD
_x000D_ Observed band size: 42 kD
_x000D_
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文献和实验该产品被引用文献
[IF=5.6] Yuyao Zhang. et al. Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody. BIOSENSORS-BASEL. 2025 Jan;15(1):47 Human
[IF=3.77] Zhan Xie. et al. A novel model of subretinal edema induced by DL-alpha aminoadipic acid. EXP EYE RES. 2023 Jan;:109388 WB Rabbit,Mouse,Rat
[IF=2.5] Zhou Zhang. et al. Acupuncture waggle needling alleviates spastic movement disorder in post-stroke rats via GLT-1 activation to modulate Glu/GABA-Gln cycle. PHYSIOL BEHAV. 2025 Nov;301:115059 WB Rat
[IF=18.027] Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX WB Human
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GLUL | Glutamine synthetase Rabbit pAb(bs-4143R)-50ul/100ul/200ul
￥1180 - 2800
