Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
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Incubation: Anti-AQP8/Aquaporin 8 Polyclonal Antibody, Unconjugated(bs-6786R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- ￥1180 - 2800
- Bioss
- bs-6786R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6786R |
| 英文名称 | Aquaporin 8 Rabbit pAb |
| 中文名称 | 水通道蛋白8抗体 |
| 英文别名 | AQP 8; AQP-8; AQP8; AQP8_HUMAN; Aquaporin 8; Aquaporin-8; Aquaporin8. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Aquaporin 8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Metabolism > Plasma Membrane > Channels |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed only in pancreas and colon. |
| 相似性 | Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Forms a water-specific channel; mercury-sensitive.plays a role in water transport across hepatocyte membranes [RGD, Feb 2006] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF=7.675] Yimeng Fan. et al. Pingwei San Ameliorates Spleen Deficiency-Induced Diarrhea through Intestinal Barrier Protection and Gut Microbiota Modulation. ANTIOXIDANTS-BASEL. 2023 May;12(5):1122 IHC Rat
[IF=6.107] Xiao-Hu Zhao. et al. Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients. ISCIENCE. 2023 May;:106831 WB Human
[IF=5.279] Qi-Le Zuo. et al. Influences of Xylitol Consumption at Different Dosages on Intestinal Tissues and Gut Microbiota in Rats. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX IHC rat
[IF=5.165] Chenhong Lin. et al. Osmotic pressure induces translocation of aquaporin-8 by P38 and JNK MAPK signaling pathways in patients with functional constipation. DIGEST LIVER DIS. 2023 Feb;: WB,IF Human
[IF=3.414] Li X et al. Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge. In loperamide-induced rats. J Ethnopharmacol. 2019 Aug 10;240:111961. WB Rat
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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AQP8 Rabbit pAb(bs-6786R)-50ul/100ul/200ul
￥1180 - 2800
