Blank control: SH-SY5Y.
_x000D_
Primary Antibody (green line): Rabbit Anti-p57 Kip2/Cdkn1c antibody (bs-0538R)
_x000D_
Dilution: 1ug/Test;
_x000D_
Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_
Dilution: 0.5ug/Test.
_x000D_
Protocol
_x000D_
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
- ￥1180 - 2800
- Bioss
- bs-0538R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0538R |
| 英文名称 | Cdkn1c Rabbit pAb |
| 中文名称 | 周期蛋白依赖激酶抑制因子1C抗体 |
| 英文别名 | Beckwith Wiedemann syndrome; BWCR; BWS; CDKI; CDKN 1C; CDKN1C; Cyclin dependent kinase inhibitor 1C; Cyclin dependent kinase inhibitor p57; KIP 2; KIP2; p57; p57 Kip2; p57 Kip 2; p57Kip2; WBS; IMAGE; CDN1C_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from rat Cdkn1c |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 35 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cell cycle inhibitors > Cip/kip Cell Biology > Cell Cycle > Cell Cycle Inhibitors > Cip / Kip Epigenetics and Nuclear Signaling > Cell cycle > Cell Cycle Inhibitors > Cip/Kip |
| 亚基 | Interacts with PCNA. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver. |
| 相似性 | Belongs to the CDI family. |
| 功能 | Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {0.2ug/test} |
Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p57 Kip2) Polyclonal Antibody, Unconjugated (bs-0538R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p57 Kip2) Polyclonal Antibody, Unconjugated (bs-0538R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Hela.
_x000D_ Primary Antibody (green line): Rabbit Anti-p57 Kip2/Cdkn1c antibody (bs-0538R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-p57 Kip2/Cdkn1c antibody (bs-0538R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Sample:
_x000D_ Lane 1: Rat Placenta tissue lysates
_x000D_ Lane 2: Rat Cerebrum tissue lysates
_x000D_ Lane 3: Human SH-SY5Y cell lysates
_x000D_ Lane 4: Human HeLa cell lysates
_x000D_ Lane 5: Human U-2 OS cell lysates
_x000D_ Lane 6: Human Jurkat cell lysates
_x000D_ Primary: Anti-Cdkn1c (bs-0538R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 35 kDa
_x000D_ Observed band size: 61 kDa
_x000D_ Lane 1: Rat Placenta tissue lysates
_x000D_ Lane 2: Rat Cerebrum tissue lysates
_x000D_ Lane 3: Human SH-SY5Y cell lysates
_x000D_ Lane 4: Human HeLa cell lysates
_x000D_ Lane 5: Human U-2 OS cell lysates
_x000D_ Lane 6: Human Jurkat cell lysates
_x000D_ Primary: Anti-Cdkn1c (bs-0538R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 35 kDa
_x000D_ Observed band size: 61 kDa
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[IF=21.3] Grigorash Bogdan B.. et al. p16High senescence restricts cellular plasticity during somatic cell reprogramming. NAT CELL BIOL. 2023 Aug;25(9):1265-1278 IF Mouse
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Cdkn1c | p57Kip2 Rabbit pAb(bs-0538R)-50ul/100ul/200ul
￥1180 - 2800
