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- bs-2146R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2146R |
| 英文名称 | HBV pre-X protein Rabbit pAb |
| 中文名称 | 乙型肝炎病毒X蛋白 (HBX) 抗体 |
| 英文别名 | HBX; pre-X protein; HB-X; pre X protein; HBV X protein; pre-X protein [Hepatitis B virus]; Hepatitis B virus pre-X protein pre X protein; |
| 产品应用 | ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Hepatitis B virus) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from Hepatitis B virus X protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Microbiology > Organism > Virus > DNA Virus > double stranded DNA Virus > Hepatitis B |
| 亚基 | Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion. |
| 亚细胞定位 | Virion membrane. |
| 翻译后修饰 | Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region.
Myristoylated. |
| 相似性 | Belongs to the orthohepadnavirus major surface antigen family. |
| 功能 | The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 |
| 应用 | 推荐稀释比例 |
| {ELISA} | {1:5000-10000} |
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting
polypeptides, core or nucleocapsid polypeptide, the viral polymerase, and the so-called “X” protein (1 ,2 ).
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
)), or control rabbit IgG (5 µl (1.0 µg/µl) from EZ ChIP kit) into each chromatin sample. (20) Incubate overnight at 4°C with horizontal rocking. (21) Add 60 µl of Protein G agarose. Rock for 1 hour at 4°C. (22) Pellet agarose by brief
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