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PAX3 Rabbit pAb(bs-1097R)-50ul

/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-1097R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1097R
    英文名称PAX3 Rabbit pAb
    中文名称配对盒基因3抗体
    英文别名CDHS; HUP 2; HUP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; Paired box protein Pax 3; Paired box protein Pax3; paired domain gene 3; paired domain gene HuP2; PAX 3; PAX3/FKHR fusion gene; Waardenburg syndrome 1; WS 1; WS1.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PAX3
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量53 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Developmental Biology > Lineage specification > Ectoderm

    Developmental Biology > Organogenesis > Nervous system development

    Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families > PAX

    Stem Cells > Lineage Markers > Ectoderm

    Stem Cells > Mesenchymal Stem Cells > Myogenesis

    Stem Cells > Neural Stem Cells > Intracellular

    Stem Cells > Neural Stem Cells > Neural Crest Stem Cells

    亚基Can bind to DNA as a homodimer or a heterodimer with PAX7. Interacts with PAXBP1; the interaction links PAX3 to a WDR5-containing histone methyltransferase complex. Interacts with DAXX.
    亚细胞定位Nucleus.
    相似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
    功能Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This protein is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAX3) Polyclonal Antibody, Unconjugated (bs-1097R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
    产品细节图片2
    Tissue/cell: rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片3
    Tissue/cell: Human esophageal carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-PAX3 Polyclonal Antibody, Unconjugated(bs-1097R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片4
    Sample:
    Brain (Mouse) Lysate at 40 ug
    Primary: Anti-PAX3 (bs-1097R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 53 kD
    Observed band size: 53 kD
    产品细节图片5
    Sample:
    Lane 1: Mouse Cerebellum tissue lysates
    Lane 2: Mouse Muscle tissue lysates
    Lane 3: Rat Cerebellum tissue lysates
    Lane 4: Rat Muscle tissue lysates
    Primary: Anti-PAX3 (bs-1097R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 53 kDa
    Observed band size: 53 kDa

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    图标文献和实验
    该产品被引用文献

    [IF={{ 4.925 }}] {Yuanyuan Chen. et al. Single-Cell Characterization of the Frizzled 5 (Fz5) Mutant Mouse and Human Persistent Fetal Vasculature (PFV). INVEST OPHTH VIS SCI. 2023 Mar;64(3):8-8} {IHC} {Human}

    [IF={{ 4.5 }}] {Dong, Changsheng, et al. "Functional Role of Cyclin-Dependent Kinase 5 in the Regulation of Melanogenesis and Epidermal Structure." Scientific Reports (Nature Publisher Group) 7 (2017): 1.} {WB} {="Mouse"}

    [IF={{ 4.4 }}] {Li Zhang. et al. Cycloleucine induces neural tube defects by reducing Pax3 expression and impairing the balance of proliferation and apoptosis in early neurulation. NEUROCHEM INT. 2024 Sep;:105861} {IHC} {Mouse}

    [IF={{ 3.8 }}] {Xu, Dan, et al. "High expression of hippocampal glutamic acid decarboxylase 67 mediates hypersensitivity of the hypothalamic-pituitary-adrenal axis in response to prenatal caffeine exposure in rats." Toxicology Letters (2017).} {IF} {=""}

    [IF={{ 3.04 }}] {Bonchak, Jonathan G., et al. "Targeting melanocyte and melanoma stem cells by 8-hydroxy-2-dipropylaminotetralin." Archives of Biochemistry and Biophysics (2014).} {IHC} {="Human"}

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    PAX3 Rabbit pAb(bs-1097R)-50ul/100ul/200ul
    ￥1180 - 2800