Blank control (blue line): Mouse spleen cells(blue).
Primary Antibody (green line): Rabbit Anti-PD-1/PE-CY7 Conjugated antibody (bs-1867R-PE-CY7)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-1867R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1867R |
| 英文名称 | PD-1 Rabbit pAb |
| 中文名称 | 程序性死亡1（CD279）抗体 |
| 英文别名 | Programmed cell death protein 1; CD279; CD279 antigen; hPD 1; hPD-1; hSLE1; PD 1; PD1; PDCD 1; PDCD1; PDCD1_HUMAN; Programmed cell death 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2. |
| 产品应用 | Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PD-1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 32 kDa |
| 实际分子量 | 55-60kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 |
Cancer > Cell Death > Apoptosis Cancer > Invasion/microenvironment > Apoptosis Cancer > Tumor immunology > CD markers Cell Biology > Apoptosis > Receptors > Death Receptors Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD |
| 亚基 | Monomer. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 |
Expressed in lymph node and 85 other tissues |
| 相似性 | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 |
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg /test} |
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文献和实验该产品被引用文献
[IF={{ 6.832 }}] {Aussel, Clotilde. et al. IL-1β primed mesenchymal stromal cells moderate hemorrhagic shock-induced organ injuries. Stem Cell Res Ther. 2021 Dec;12(1):1-16} {FCM} {Rat}
[IF={{ 6.639 }}] {Ling Ding. et al. Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity. Cancers. 2021 Jan;13(22):5729} {WB} {Human}
[IF={{ 4.657 }}] {He, Xiaojing. et al. Inhibition of PD-1 Alters the SHP1/2-PI3K/Akt Axis to Decrease M1 Polarization of Alveolar Macrophages in Lung Ischemia–Reperfusion Injury. INFLAMMATION. 2022 Nov;:1-16} {WB} {Rat}
[IF={{ 4.068 }}] {Li Wang. et al. Radiotherapy upregulated immune checkpoints contribute to the development of second primary OSCC. ORAL DIS. 2023 May;:} {WB} {Rat}
[IF={{ 4 }}] {Xinwen Dong. et al. Fecal microbiota transplantation alleviates cognitive impairment by improving gut microbiome composition and barrier function in male rats of traumatic brain injury following gas explosion. FRONT MICROBIOL. 2024 Nov;15:} {IHC,WB} {Rat}
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PD-1 Rabbit pAb(bs-1867R)-50ul/100ul/200ul
￥1180 - 2800
