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CYPA Rabbit pAb(bs-5912R)-50ul

/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-5912R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-5912R
    英文名称CYPA Rabbit pAb
    中文名称亲环蛋白（亲环素）PPIA抗体
    英文别名Cyclophilin A; CyclophilinA; Cyclosporin A binding protein; Cyclosporin A-binding protein; CYPA; CYPH; Peptidyl prolyl cis trans isomerase A; Peptidyl-prolyl cis-trans isomerase A; Peptidylprolyl isomerase A; PPIA; PPIA protein; PPIA_HUMAN; PPIase A; Rotamase A; RotamaseA; T cell cyclophilin.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Pig, Cow, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Cyclophilin A.
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量18 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Immunology > Immune Interventions > Transplantation

    Signal Transduction > Signaling Pathway > Calcium Signaling

    亚细胞定位Cytoplasm.
    相似性Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
    Contains 1 PPIase cyclophilin-type domain.
    功能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity (1). Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins (2,3). Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans (4,5). CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain (6). CyPA is incorporated into the virion of the type 1 H‌‌uman immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication (7,8). Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10).

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1μg/Test}

     

    产品细节图片1
    Sample:
    Cerebrum (Rat) Lysate at 40 ug
    Hela(Human) Cell Lysate at 30 ug
    Primary: Anti-CYPA (bs-5912R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 18 kD
    Observed band size: 17/18 kD
    产品细节图片2
    Sample:
    NIH/3T3(Mouse) Cell Lysate at 30 ug
    A431(Human) Cell Lysate at 30 ug
    Primary: Anti-CYPA (bs-5912R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 18 kD
    Observed band size: 18 kD
    产品细节图片3
    Tissue/cell: rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated(bs-5912R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片4
    Blank control(blue): Hela(fixed with 2% P‌‌araformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
    Primary Antibody:Rabbit Anti- CYPA antibody(bs-5912R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    产品细节图片5
    Tissue/cell: rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated(bs-5912R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片6
    Sample:
    Lane 1: Mouse Thymus tissue lysates
    Lane 2: Mouse Lymph node tissue lysates
    Lane 3: Mouse Lung tissue lysates
    Lane 4: Mouse NIH/3T3 cell lysates
    Lane 5: Rat Thymus tissue lysates
    Lane 6: Rat Lymph node tissue lysates
    Lane 7: Rat Lung tissue lysates
    Lane 8: Human Hela cell lysates
    Lane 9: Human PANC-1 cell lysates
    Lane 10: Human Molt-4 cell lysates
    Lane 11: Human HUVEC cell lysates
    Lane 12: Human THP-1 cell lysates
    Primary: Anti- CYPA (bs-5912R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 18 kDa
    Observed band size: 18 kDa

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    [IF={{ 3.58 }}] {Kang, Taewook, et al. "Large-scale analysis of posttranslational modifications in the hippocampus of patients with Alzheimer’s disease using pI shift and label-free quantification without enrichment." Analytical and Bioanalytical Chemistry (2014): 1-14.} {WB} {="Human"}

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    CYPA Rabbit pAb(bs-5912R)-50ul/100ul/200ul
    ￥1180 - 2800