Sample: Mcf-7 Cell Lysate at 40 ug
Primary: Anti- Mesothelin (bs-0300R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 62 kD
- ￥1180 - 2800
- Bioss
- 北京
- bs-0300R
- 2025年10月15日
- WB=1:500-2000,Elisa=1:500-1000,Flow Cyt=1μg/Test,
- Rabbit
- Human,
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide derived from human Mesothelin
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 保存条件：
Store at -20 °C for one year.
- 克隆性：
单克隆
- 标记物：
无
- 适应物种：
Human,
- 保质期：
1年
- 目录编号：
bs-0300R
- 库存：
999
- 供应商：
北京博奥森生物技术有限公司
- 宿主：
Rabbit
- 应用范围：
WB=1:500-2000,Elisa=1:500-1000,Flow Cyt=1μg/Test,
- 浓度：
1mg/ml
- 抗体英文名：
Mesothelin
- 抗体名：
间皮素抗体
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.00 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.00 |
| 规格： | 200ul | 产品价格： | ￥2800.00 |
| 产品编号 | bs-0300R |
| 英文名称 | Mesothelin Rabbit pAb |
| 中文名称 | 间皮素抗体 |
| 英文别名 | MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN. |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Mesothelin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 64 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers > Tumor antigens Cancer > Tumor immunology > Tumor-associated antigens Tags & Cell Markers > Cell Type Markers > Epi / Endo-thelial Tags & Cell Markers > Cell Type Markers > Tumor Associated |
| 亚基 | Interacts with MUC16. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.
Megakaryocyte-potentiating factor: Secreted. Isoform 3: Secreted. |
| 组织特异性 | Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). |
| 翻译后修饰 | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. |
| 相似性 | Belongs to the mesothelin family. |
| 功能 | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Mesothelin(MSLN)may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma. malignant tumors, such as a skin lump, ovary cancer and the pancreas cancer...etc..Mainly used for the research of the malignant tumor. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Blank control: Hela(blue).
Primary Antibody: Rabbit Anti-Mesothelin/AF647 Conjugated antibody (bs-0300R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-0300R-AF647, 0.2μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
Primary Antibody: Rabbit Anti-Mesothelin/AF647 Conjugated antibody (bs-0300R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-0300R-AF647, 0.2μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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anti-Mesothelin
￥1180 - 2800
