MSLN Rabbit pAb(bs-0300R)-50ul/100ul/200ul产品图
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MSLN Rabbit pAb(bs-0300R)-50ul

/100ul/200ul
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  • ¥1180 - 2800
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  • bs-0300R
  • 2026年10月13日
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-0300R
    英文名称Mesothelin Rabbit pAb
    中文名称间皮素抗体
    英文别名MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN.
    产品应用WB=1:500-2000, ELISA=1:5000-10000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human (Mouse, Rat)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Mesothelin
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量64 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Tumor biomarkers > Tumor antigens

    Cancer > Tumor immunology > Tumor-associated antigens

    Tags & Cell Markers > Cell Type Markers > Epi / Endo-thelial

    Tags & Cell Markers > Cell Type Markers > Tumor Associated

    亚基Interacts with MUC16.
    亚细胞定位Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus._x000D_
    Megakaryocyte-potentiating factor: Secreted._x000D_
    Isoform 3: Secreted.
    组织特异性Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
    翻译后修饰Both MPF and the cleaved form of mesothelin are N-glycosylated.
    _x000D_ Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
    相似性Belongs to the mesothelin family.
    功能Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Mesothelin(MSLN)may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma. malignant tumors, such as a skin lump, ovary cancer and the pancreas cancer...etc..Mainly used for the research of the malignant tumor.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {ELISA}{1:5000-10000}

     

    产品细节图片1
    Sample: Mcf-7 Cell Lysate at 40 ug
    _x000D_ Primary: Anti- Mesothelin (bs-0300R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 64 kD
    _x000D_ Observed band size: 62 kD
    _x000D_
    产品细节图片2
    Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
    _x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), _x000D_ Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green)._x000D_
    产品细节图片3
    Blank control: Hela(blue).
    Primary Antibody: Rabbit Anti-Mesothelin/AF647 Conjugated antibody (bs-0300R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
    _x000D_ _x000D_ Protocol
    _x000D_ The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-0300R-AF647, 0.2μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed._x000D_

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    图标文献和实验
    该产品被引用文献

    [IF=34.9] Zhao Zhipeng. et al. A collagenase nanogel backpack improves CAR-T cell therapy outcomes in pancreatic cancer. NAT NANOTECHNOL. 2025 May;:1-11 FC Human

    相关实验
    • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

      = NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

    • 细胞因子试剂盒免费实验代测

      T 2000/3000 进口分装 F0290 TNF 相关凋亡诱导配体 (TRAIL/APO2 Ligand) ELISA 48T/96T 2000/3000 进口分装 F0291 血栓前体蛋白(TpP) ELISA 48T/96T 2000/3000 进口分装 F0300 尿激酶型纤溶酶原激活因子(uPA) ELISA 48T/96T 2000/3000 进口分装 F0301 尿激酶型纤溶酶原激活因子受体(uPA-R) ELISA 48T

    • 细胞因子试剂

      T 2000/3000 进口分装 F0290 TNF 相关凋亡诱导配体 (TRAIL/APO2 Ligand) ELISA 48T/96T 2000/3000 进口分装 F0291 血栓前体蛋白(TpP) ELISA 48T/96T 2000/3000 进口分装 F0300 尿激酶型纤溶酶原激活因子(uPA) ELISA 48T/96T 2000/3000 进口分装 F0301 尿激酶型纤溶酶原激活因子受体(uPA-R) ELISA 48T

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    MSLN Rabbit pAb(bs-0300R)-50ul/100ul/200ul
    ¥1180 - 2800