Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-NRSF (bs-2590R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
- ￥1180 - 2800
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- bs-2590R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2590R |
| 英文名称 | NRSF Rabbit pAb |
| 中文名称 | 神经元抑制蛋白抗体 |
| 英文别名 | RE1-Silencing Transcription Factor; NRSF; Neural-Restrictive Silencer Factor; XBR; Neuron Restrictive Silencer Factor; Repressor Binding To The X2 Box; X2 Box Repressor; REST_HUMAN |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, IP=1:50-200 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NRSF |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 121 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Co-factors Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Neuroscience > Neurology process > Neurogenesis |
| 亚基 | Interacts with SIN3A, SIN3B and RCOR1. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. |
| 相似性 | Contains 9 C2H2-type zinc fingers. |
| 功能 | Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {IP} | {1:50-200} |
Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(bs-2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(bs-2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
NRSF was immunoprecipitated from human hela cells lysate with bs-2590R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: bs-2590R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: bs-2590R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO NRSF (Human) Cell Lysate at 30 ug
Primary: Anti- NRSF (bs-2590R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
Hela(Human) Cell Lysate at 30 ug
Hela KO NRSF (Human) Cell Lysate at 30 ug
Primary: Anti- NRSF (bs-2590R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
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文献和实验该产品被引用文献
[IF={{ 9.889 }}] {Buqing Yeet al. The chromatin remodeler SRCAP promotes self‐renewal of intestinal stem cells. EMBO J . 2020 Jul 1;39(13):e103786.} {IHC,IP,IF} {mouse}
[IF={{ 6.6 }}] {Dongze Zhang. et al. Hydrogen Peroxide-Induced Re-Expression of Repressor Element 1-Silencing Transcription Factor Contributes to Cardiac Vagal Dysfunction in Type 2 Diabetes Mellitus. ANTIOXIDANTS-BASEL. 2025 May;14(5):588} {WB} {Mouse,Rat}
[IF={{ 5.3 }}] {Christopher J. O'Connell. et al. Transcriptomic dynamics governing serotonergic dysregulation in the dorsal raphe nucleus following mild traumatic brain injury. EXP NEUROL. 2024 Apr;374:114695} {IHC} {Mouse}
[IF={{ 2.472 }}] {Zhu NW et al. Possible mechanisms of lycopene amelioration of learning and memory impairment in rats with vascular dementia. Neural Regen Res. 2020 Feb;15(2):332-341.} {WB,IHC} {Rat}
[IF={{ 11.8 }}] {Kojiro Ishibashi. et al. Astrocyte-induced mGluR1 activates human lung cancer brain metastasis via glutamate-dependent stabilization of EGFR. DEV CELL. 2024 Feb 02} {ICC} {Human}
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NRSF Rabbit pAb(bs-2590R)-50ul/100ul/200ul
￥1180 - 2800
