EDN1 | PPET1 Rabbit pAb(bs-0188R)-50ul/100ul/200ul产品图
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EDN1 | PPET1 Rabbit pAb(bs-018

8R)-50ul/100ul/200ul
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  • bs-0188R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-0188R
    英文名称PPET1 Rabbit pAb
    中文名称内皮素-1前体抗体
    英文别名EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat (Human)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Big endothelin-1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量22 kDa
    实际分子量24-27kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Response to hypoxia

    Cardiovascular > Vasculature > Endothelium

    Cardiovascular > Vasculature > Vasoconstriction

    Metabolism > Pathways and Processes > Metabolism processes > Hypoxia

    Metabolism > Types of disease > Cancer

    Signal Transduction > Growth Factors/Hormones > Hormones

    亚细胞定位Secreted.
    组织特异性Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
    相似性Belongs to the endothelin/sarafotoxin family.
    功能Endothelins are endothelium-derived vasoconstrictor peptides.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.Molecular weight: 2491.9Molecular formula: C109H159N25O32S5CAS: 117399-94-7MDL: MFCD00133305Reconstitution: 1mM (1mg/0.4013mL)

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Big endothelin-1) Polyclonal Antibody, Unconjugated (bs-0188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    Tissue/cell: Rat lung tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti- Big endothelin-1 Polyclonal Antibody, Unconjugated(bs-0118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片3
    Tissue/cell: Rat stomach tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti- Big endothelin-1 Polyclonal Antibody, Unconjugated(bs-0118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片4
    Sample:
    _x000D_ Lane 1: Testis (Mouse) Lysate at 40 ug
    _x000D_ Lane 2: Testis (Rat) Lysate at 40 ug
    _x000D_ Primary:
    _x000D_ Anti-Big endothelin-1 (bs-0188R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 24 kD
    _x000D_ Observed band size: 26 kD

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    图标文献和实验
    该产品被引用文献

    [IF=3.164] Riggs JL et al. Intermittent Hypoxia Exacerbates Increased Blood Pressure in Rats with Chronic Kidney Disease JL Riggs, CE Pace, HH Ward, LV Gonzalez Bosc… - American Journal of …, 2018Am J Physiol Renal Physiol. 2018 Oct 1;315(4):F927-F941. IHC Rat

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    EDN1 | PPET1 Rabbit pAb(bs-0188R)-50ul/100ul/200ul
    ￥1180 - 2800