ox-LDL Rabbit pAb(bs-1698R)-50ul/100ul/200ul
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ox-LDL Rabbit pAb(bs-1698R)-50

ul/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-1698R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-1698R
    英文名称ox-LDL Rabbit pAb
    中文名称氧化低密度脂蛋白抗体
    英文别名ox-LDL: LDL(Copper oxidized); Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein.
    中文别名氧化型低密度脂蛋白;
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Rabbit
    免疫原Full length protein from human plasma
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量31 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚细胞定位Secreted.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{2ug/Test}

     

    ox-LDL Rabbit pAb(bs-1698R)-50
    P‌‌araformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (bs-1698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    ox-LDL Rabbit pAb(bs-1698R)-50
    Blank control: A431.
    Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (bs-1698R)
    Dilution: 3μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF647
    Dilution: 3μg /test.
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    ox-LDL Rabbit pAb(bs-1698R)-50
    Blank control:HepG2.
    Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (bs-1698R)
    Dilution: 2ug/Test;
    Secondary Antibody : Goat anti-rabbit IgG-FITC
    Dilution: 0.5ug/Test.
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 7.396 }}] {Takuya Tsumita. et al. The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells. INT J CANCER. 2022 May 24} {IF} {Mouse}

    [IF={{ 6.706 }}] {Qing Li. et al. Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets. NUTRIENTS. 2023 Jan;15(4):832} {IHC} {Mouse}

    [IF={{ 4.473 }}] {Xiaosu Song. et al. Long noncoding RNA THRIL promotes foam cell formation and inflammation in macrophages. CELL BIOL INT. 2022 Oct;:} {Other} {Other}

    [IF={{ 4.39 }}] {Wen, Song, et al. "OxLDL-targeted iron oxide nanoparticles for in vivo MRI detection of perivascular carotid collar induced atherosclerotic lesions in ApoE-deficient mice." Journal of Lipid Research 53.5 (2012): 829-838.} {IHC} {="Mouse"}

    [IF={{ 4.164 }}] {Fen Gao. et al. Angiotensin II induces the expression of periostin to promote foam cell formation in oxLDL-treated macrophages. Int J Cardiol. 2022 Jan;347:46} {ELISA} {Human}

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    文献支持
    ox-LDL Rabbit pAb(bs-1698R)-50ul/100ul/200ul
    ¥1180 - 2800