Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (bs-3155R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-3155R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
SLC39A10 Rabbit pAb(bs-19828R)-50ul/100ul/200ul
￥1180
CASPR Rabbit pAb, BF594 conjugated(bs-11128R-BF594)-100ul
￥2980HSD17B6 Rabbit pAb, PerCP conjugated(bs-6592R-PerCP)-100ul
￥2980Claudin 15 Rabbit pAb, Biotin conjugated(bs-13753R-Bio)-100ul
￥2980BCL2L1 Rabbit pAb, BF488 conjugated(bs-1336R-BF488)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3155R |
| 英文名称 | phospho-GCN2 (Thr899) Rabbit pAb |
| 中文名称 | 磷酸化蛋白激酶GCN2蛋白抗体 |
| 英文别名 | GCN2(phospho T899); p-GCN2(phospho T899); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2. |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 189 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 组织特异性 | Widely expressed. |
| 翻译后修饰 | Autophosphorylated on threonine residues. |
| 相似性 | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. |
| 功能 | Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-Phospho-GCN2 (Thr899) antibody (bs-3155R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line):Rabbit Anti-Phospho-GCN2 (Thr899) antibody (bs-3155R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 5.58 }}] {Lehman, Stacey L., Sandra Ryeom, and Constantinos Koumenis. "Signaling through alternative Integrated Stress Response pathways compensates for GCN2 loss in a mouse model of soft tissue sarcoma." Scientific Reports 5 (2015).} {WB} {="Mouse"}
[IF={{ 4.9 }}] {Zikang Xing. et al. IDO1 Inhibitor RY103 Suppresses Trp-GCN2-Mediated Angiogenesis and Counters Immunosuppression in Glioblastoma. PHARMACEUTICS. 2024 Jul;16(7):870} {WB} {Mouse,Human}
[IF={{ 25.269 }}] {Changzheng Li. et al. Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis. CELL STEM CELL. 2022 Jul;29:1119} {WB} {Mouse}
相关实验
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer
技术资料暂无技术资料 索取技术资料
文献支持
phospho-GCN2 (Thr899) Rabbit pAb(bs-3155R)-50ul/100ul/200ul
￥1180 - 2800
