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- bsm-1622M
- 2026年10月13日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-1622M |
| 英文名称 | AFP(A4) Mouse mAb |
| 中文名称 | 甲胎蛋白单克隆抗体(检测) |
| 英文别名 | alpha Fetoprotein; alpha-fetoprotein; alpha 1 fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN. |
| 产品应用 | ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | AFP protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 65 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 研究领域 | Cancer > Tumor biomarkers > Tumor antigens Cardiovascular > Blood > Serum Proteins Developmental Biology > Organogenesis > Gut development > Liver development Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Cardiovascular ELISA kits |
| 亚基 | Dimeric and trimeric forms have been found in addition to the monomeric form. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Plasma. Synthesized by the fetal liver and yolk sac. |
| 翻译后修饰 | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
| 相似性 | Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. |
| 功能 | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. |
| 应用 | 推荐稀释比例 |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF=6.641] Zhao J et al. A SERS-based immunoassay for the detection of α-fetoprotein using AuNS@ Ag@ SiO2 core-shell nanostars. Journal of Materials Chemistry C. 2019. Other
[IF=6.45] Lin, Dajie, et al. "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." Biosensors and Bioelectronics (2014). Other =""
[IF=6.277] Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019. Other
[IF=5.88] Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016). Other =""
[IF=5.83] Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415. Other ="Human"
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
. Use gloves and aerosol barrier tips. Linearize the plasmid and check the digest. Phenol extract. Extract twice with chloroform:isoamyl alcohol (24:1) Ethanol precipitate ( 1/2 vol 7.5 M NH4 OAc + 2.5 vol 100
Hybrid Resistance by Mouse NK Cells In Vitro
A purified NK cells to kill H2d target cells can be reversed by adding MAb reagents that bind to either Ly49A or H2-Dd . We and others have extended these early findings to the Ly49C and I subsets of NK cells, and have recently detected positive signaling
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