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- bs-0194R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0194R |
| 英文名称 | GRP94 Rabbit pAb |
| 中文名称 | 葡萄糖调节蛋白94抗体 |
| 英文别名 | HSP gp96; heat shock protein gp96 precursor; 94 kDa glucose regulated protein; ECGP; Endoplasmin; Endothelial cell(HBMEC) glycoprotein; Glucose regulated protein 94; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; Heat shock protein 90 kDa beta member 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; Tumor rejection antigen 1; Tumor rejection antigen gp96; ENPL_HUMAN; Endoplasmin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GRP94 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 86 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Signal Transduction > Protein Trafficking > ER Proteins Tags & Cell Markers > Subcellular Markers > Organelles > ER |
| 亚基 | Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP (By similarity). Interacts with TLR4 and TLR9, but not with TLR3. |
| 亚细胞定位 | Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 相似性 | Belongs to the heat shock protein 90 family. |
| 功能 | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-0194P is one synthetic peptide derived from human GRP94. Glucose regulated protein 94 (GRP 94) is a resident protein of the endoplasmic reticulum (ER) and is induced by the accumulation of unfolded proteins suggesting that it might associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP 94 and other resident ER proteins including GRP 78 and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. GRP 94 is also a low affinity, high capacity calcium binding protein, though it's role, if any, in calcium regulation is not understood. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP94) Polyclonal Antibody, Unconjugated (bs-0194R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP94) Polyclonal Antibody, Unconjugated (bs-0194R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP94) Polyclonal Antibody, Unconjugated (bs-0194R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP94) Polyclonal Antibody, Unconjugated (bs-0194R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-GRP94/HSP gp96 Polyclonal Antibody, Unconjugated(bs-0194R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-GRP94/HSP gp96 Polyclonal Antibody, Unconjugated(bs-0194R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Sample: Placenta (Mouse) Lysate at 30 ug
_x000D_ Primary: Anti- GRP94 (bs-0194R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
_x000D_ Predicted band size: 78 kD
_x000D_ Observed band size: 100 kD
_x000D_
_x000D_ Primary: Anti- GRP94 (bs-0194R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
_x000D_ Predicted band size: 78 kD
_x000D_ Observed band size: 100 kD
_x000D_
Blank control: Hela.
_x000D_ Primary Antibody (green line): Rabbit Anti-GRP94 antibody (bs-0194R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-GRP94 antibody (bs-0194R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Sample:
_x000D_ Lane 1: Mouse Spleen Lysates
_x000D_ Lane 2: Mouse NIH/3T3 cell Lysates
_x000D_ _x000D_ _x000D_ _x000D_ Primary: Anti-GRP94 (bs-0194R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 86kDa
_x000D_ Observed band size: 100kDa
_x000D_ Lane 1: Mouse Spleen Lysates
_x000D_ Lane 2: Mouse NIH/3T3 cell Lysates
_x000D_ _x000D_ _x000D_ _x000D_ Primary: Anti-GRP94 (bs-0194R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 86kDa
_x000D_ Observed band size: 100kDa
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP94) Polyclonal Antibody, Unconjugated (bs-0194R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_
_x000D_
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP94) Polyclonal Antibody, Unconjugated (bs-0194R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_
Blank control (Black line): A431 (Black).
_x000D_ Primary Antibody (green line): Rabbit Anti-EphB2 antibody (bs-0194R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 3μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-EphB2 antibody (bs-0194R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 3μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Blank control: A431.
_x000D_ Primary Antibody (green line): Rabbit Anti-GRP94 antibody (bs-0194R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody: Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
_x000D_ Primary Antibody (green line): Rabbit Anti-GRP94 antibody (bs-0194R)
_x000D_ Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody: Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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[IF=5.168] Feiyang Ma. et al. New insights into the interaction between duodenal toxicity and microbiota disorder under copper exposure in chicken: Involving in endoplasmic reticulum stress and mitochondrial toxicity. CHEM-BIOL INTERACT. 2022 Oct;366:110132 WB Chicken
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[IF=4.522] Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25. WB,ICC Mouse
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HSP90B1 | gp96/GRP94 Rabbit pAb(bs-0194R)-50ul/100ul/200ul
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