Blank control（black line）:A431.
_x000D_
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R)
_x000D_
Dilution:1ug/Test;
_x000D_
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
_x000D_
Dilution: 0.5ug/Test.
_x000D_
Isotype control（orange line）: Normal Rabbit IgG
_x000D_
Protocol
_x000D_
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
- ￥1180 - 2800
- Bioss
- bs-1253R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1253R |
| 英文名称 | AQP3 Rabbit pAb |
| 中文名称 | 水通道蛋白-3抗体 |
| 英文别名 | AQP 3; AQP3; Aquaporin 3(GIL blood group); Aquaporin 3(Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood group; AQP3_MOUSE. |
| 产品应用 | Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse AQP3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 32 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes. |
| 相似性 | Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| 功能 | Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
Blank control: Hela.
_x000D_ Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R)
_x000D_ Dilution: 3μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody: Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 3μg /test.
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
_x000D_ Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R)
_x000D_ Dilution: 3μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody: Goat anti-rabbit IgG-AF647
_x000D_ Dilution: 3μg /test.
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Aqp3 Rabbit pAb(bs-1253R)-50ul/100ul/200ul
￥1180 - 2800
