Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated(bs-1207R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
- ￥1180 - 2800
- Bioss
- bs-1207R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1207R |
| 英文名称 | GLUT3 Rabbit pAb |
| 中文名称 | 葡萄糖转运蛋白3抗体 |
| 英文别名 | facilitated glucose transporter member 3; Glucose Transporter GLUT3; FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT-3; GTR3_HUMAN; SLC2A3; Solute Carrier Family 2(Facilitated Glucose Transporter) Member 3; Solute carrier family 2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GLUT3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 54 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Cell Biology > Other Antibodies > Other Antibodies Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Plasma Membrane > Channels |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Melanosome. |
| 组织特异性 | Highly expressed in brain. Expressed in many tissues. |
| 翻译后修饰 | he cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested by cathepsin CTSL within endosomes. {ECO:0000269|PubMed:17134730}. |
| 相似性 | Belongs to the coronaviruses spike protein family. {ECO:0000305}. |
| 功能 | Facilitative glucose transporter. Probably a neuronal glucose transporter. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | May act as a glucose transporter in neurons; may mediate increasd glucose uptake in response to neuronal injury. Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis. Alternative names: FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT3; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, FITC conjugated(bs-1207R-FITC) 1:100, 60 minutes at 37°C;
Excitation wavelength: 488nm; Emission wavelength:519nm
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, FITC conjugated(bs-1207R-FITC) 1:100, 60 minutes at 37°C;
Excitation wavelength: 488nm; Emission wavelength:519nm
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated(bs-1207R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated(bs-1207R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
293T(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-bs-1207R (bs-1207R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 63 kD
293T(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-bs-1207R (bs-1207R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 63 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
293T (Human) Cell Lysate at 30 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
Primary: Anti-GLUT3 (bs-1207R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 50/63 kD
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
293T (Human) Cell Lysate at 30 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
Primary: Anti-GLUT3 (bs-1207R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 50/63 kD
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文献和实验该产品被引用文献
[IF={{ 9.7 }}] {Ziyi Zhong. et al. Tumor-associated macrophages drive glycolysis through the IL-8/STAT3/GLUT3 signaling pathway in pancreatic cancer progression. CANCER LETT. 2024 Apr;588:216784} {WB,IHC} {Human,Mouse}
[IF={{ 7.5 }}] {Ling Hao. et al. 27-Hydroxycholesterol impairs learning and memory ability via decreasing brain glucose uptake mediated by the gut microbiota. BIOMED PHARMACOTHER. 2023 Dec;168:115649} {IF} {Mouse}
[IF={{ 6.7 }}] {Cui-ling Jia. et al. Rosmarinic acid Liposomes Suppress Ferroptosis in Ischemic Brain via Inhibition of TfR1 in BMECs. PHYTOMEDICINE. 2024 Jun;:155835} {WB} {Mouse}
[IF={{ 5.47 }}] {Yan, Yu-E., et al. ʺSignificant Reduction of the GLUT3 Level, but not GLUT1 Level, Was Observed in the Brain Tissues of Several Scrapie Experimental Animals and Scrapie-Infected Cell Lines.ʺ Molecular neurobiology (2013): 1-14.} {WB} {=""}
[IF={{ 5.076 }}] {Hu C et al. Placentae for Low Birth Weight Piglets Are Vulnerable to Oxidative Stress, Mitochondrial Dysfunction, and Impaired Angiogenesis. Oxid Med Cell Longev. 2020 May 25;2020:8715412.} {WB} {Pig}
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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GLUT3 Rabbit pAb(bs-1207R)-50ul/100ul/200ul
￥1180 - 2800
