Sample:
Lane1:DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-FLAG Tag (bs-0879R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
- ￥580 - 4680
- Bioss
- bs-0879R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 文献和实验
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
100ul/500ul/200ul/1000ul
| 规格： | 100ul | 产品价格： | ￥580.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥2280.0 |
| 规格： | 200ul | 产品价格： | ￥980.0 |
| 规格： | 1000ul | 产品价格： | ￥4680.0 |
| 产品编号 | bs-0879R |
| 英文名称 | FLAG Tag (CT) Rabbit pAb |
| 中文名称 | FLAG Tag标签（C端）抗体 |
| 英文别名 | DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag. |
| 产品应用 | WB=1:5000-50000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Flag Tag peptide |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Epitope Tags > DDDDK Tag |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:5000-50000} |
| {ELISA} | {1:1000-5000} |
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文献和实验该产品被引用文献
[IF={{ 5.714 }}] {Mengmeng Zhao. et al. Interferon inducible porcine 2′, 5′-oligoadenylate synthetase like-1 protein limits porcine reproductive and respiratory syndrome virus 2 infection via the MDA5-mediated interferon-signaling pathway. INT IMMUNOPHARMACOL. 2022 Oct;111:109151} {IP,WB} {Monkey,Pig}
[IF={{ 5.6 }}] {Xi Zhao. et al. Immune Rejection Mediated by prf1 and gzmb Affects the Colonization of Fat Greenling (Hexagrammos otakii) Spermatogonia in Heterotransplantation. INT J MOL SCI. 2024 Jan;25(10):5157} {Co-IP} {Fish}
[IF={{ 5.52 }}] {Zhang, Zhenfeng, et al. "Human Bocavirus NP1 Inhibits IFN-?? Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter."The Journal of Immunology 189.3 (2012): 1144-1153.} {Other} {=""}
[IF={{ 4.7 }}] {Ming Luo. et al. Fecal Microbiota Transplantation Alleviates Cirrhotic Portal Hypertension in Rats via Butyrate-Mediated HDAC3 Inhibition and PI3K/Akt/eNOS Signaling Regulation. EUR J PHARMACOL. 2025 May;:177781} {CoIP} {Rat}
[IF={{ 3.636 }}] {Mengmeng Zhao. et al. Molecular cloning and functional analysis of Macaca mulatta STING. Dev Comp Immunol. 2022 Feb;127:104296} {WB} {Monkey}
相关实验
蛋白抗体将目的蛋白沉淀下来？如果能直接用目的蛋白的抗体，那正是我所期望的，试验简单啊。回答：1、做Co－IP时是否选用Tag的抗体不是主要的，重要的是你用的抗体是否可以做IP以及IP的效果如何。所以说，如果你目标蛋白的抗体可以用于IP，且效 果好，就不需要顾及Tag的问题。单就Co-IP或IP而言，goat抗体最好，mouse次之，rabbit不是太好，这里好坏的重要标准之一是 Western blot时重轻链信号的干扰问题.2、抗体能用就不需要标签。3、这个没有标准答案，只有做了才知道。理论上都是可行
Studies of the Ubiquitin Proteasome System
with Nonradioactive Tag (Biotin) Alternate Protocol 10: Generation of Tagged Ubiquitin Expression Plasmids Basic Protocol 18: Immunoblotting with Anti‐Ubiquitin Generation and Purification of Rabbit Anti‐Ubiquitin Antibodies Basic Protocol 19: Generation
that can lead to artifacts. The advantage to using exogenously expressed tagged proteins is the generally high specificity of the anti-tag antibody and the ease with which a negative control can be performed in which the exogenous protein is lacking.
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文献支持
FLAG Tag (CT) Rabbit pAb(bs-0879R)-100ul/500ul/200ul/1000ul
￥580 - 4680
