Tissue/cell: rat myocardium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated(bs-0630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-0630R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0630R |
| 英文名称 | Angiotensin II Type 1 Receptor Rabbit pAb |
| 中文名称 | 血管紧张素Ⅱ-1型受体抗体 |
| 英文别名 | AG2S; Agtr 1; Agtr1; Agtr1a; AGTR1B; Angiotensin II receptor type 1; Angiotensin II type 1 receptor; AT-1B; AT-1r; AT1; At1a; AT1AR; AT1B; AT1BR; AT2R1; AT2R1A; AT2R1B; HAT1R; Type 1 angiotensin II receptor; AGTR1_HUMAN; Type-1 angiotensin II receptor; Angiotensin II type-1 receptor; AT1R; Ang II; Angiotensin II receptor type 1; Angiotensin II type 1 receptor; Angiotensin receptor 1; Angiotensin receptor 1B; AT 1B; AT1; AT1R. |
| 中文别名 | 血管紧张素II 1型受体; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human AT1R |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 39 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Cancer > Invasion/microenvironment > Angiogenesis > Angiogenic growth factors Cardiovascular > Blood > Blood Pressure regulation Metabolism > Types of disease > Cancer |
| 亚基 | Interacts with MAS1 (Probable). Interacts with ARRB1. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Liver, lung, adrenal and adrenocortical adenomas. |
| 翻译后修饰 | C-terminal Ser or Thr residues may be phosphorylated. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least five transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated(bs-0630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated(bs-0630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample: Liver (mouse) Lysate at 40 ug
Primary: Anti- Angiotensin II Type 1 Receptor (bs-0630R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 36kD
Primary: Anti- Angiotensin II Type 1 Receptor (bs-0630R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 36kD
Sample: HepG2 (human)cell Lysate at 40 ug
Primary: Anti- Angiotensin II Type 1 Receptor (bs-0630R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 49kD
Primary: Anti- Angiotensin II Type 1 Receptor (bs-0630R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 49kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (bs-0630R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (bs-0630R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 4.996 }}] {Cong Changsheng. et al. Renin-angiotensin system inhibitors mitigate radiation pneumonitis by activating ACE2-angiotensin-(1–7) axis via NF-κB/MAPK pathway. SCI REP-UK. 2023 May;13(1):1-11} {WB} {Mouse}
[IF={{ 3.743 }}] {Chen Q et al. Sini decoction ameliorates sepsis-induced acute lung injury via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway. Biomed Pharmacother. 2019 Jul;115:108971.} {WB} {Mouse&Human}
[IF={{ 3.234 }}] {Liu J et al. Sini decoction alleviates E. coli induced acute lung injury in mice via equilibrating ACE-AngII-AT1R and ACE2-Ang-(1-7)-Mas axis.Life Sci. 2018 Sep 1;208:139-148.} {WB} {Mouse}
[IF={{ 2.5 }}] {Lei Li. et al. Arginine vasopressin and angiotensin II coregulate Aquaporin 2 expression in M-1 cells. BIOCHEM BIOPH RES CO. 2025 Jan;745:151256} {IF} {Mouse}
[IF={{ 14.224 }}] {Hongxu Bao. et al. Exposure to Real-Ambient Particulate Matter Induced Vascular Hypertrophy by Activation of PDGFRβ. J HAZARD MATER. 2023 Feb;:130985} {WB} {Human}
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Angiotensin II Type 1 Receptor Rabbit pAb(bs-0630R)-50ul/100ul/200ul
￥1180 - 2800
