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- bs-1926R
- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1926R |
| 英文名称 | UCP-2 Rabbit pAb |
| 中文名称 | 线粒体脱偶连蛋白2抗体 |
| 英文别名 | Uncoupling Protein-2; UCP2; UCP 2; BMIQ4; Mitochondrial uncoupling protein 2; SLC25A8; UCPH; Uncoupling protein 2; Uncoupling protein 2 mitochondrial proton carrier; Solute carrier family 25 member 8; UCP2_MOUSE. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse UCP-2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 34 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy Metabolism > Types of disease > Cancer Metabolism > Types of disease > Obesity |
| 亚基 | Acts as a dimer forming a proton channel (By similarity). |
| 亚细胞定位 | Mitochondrion inner membrane; Multi-pass membrane protein. |
| 组织特异性 | Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle. |
| 相似性 | Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. |
| 功能 | UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg /test} |
Lane 1: Stomach (Rat) Lysate at 40 ug
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 3: SiHa (Human) Cell Lysate at 30 ug
Lane 4: DU145 (Human) Cell Lysate at 30 ug
Lane 5: RAW264.7 (Mouse) Cell Lysate at 30 ug
Lane 6: Liver (Mouse) Lysate at 40 ug
Primary: Anti-UCP-2 (bs-1926R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Primary Antibody (green line): Rabbit Anti-UCP-2 antibody (bs-1926R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% Paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验[IF={{ 5.988 }}] {Lei Zhao. et al. Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities. FRONT PHARMACOL. 2022; 13: 933669} {WB} {Mouse}
[IF={{ 5.469 }}] {Tidwell, Tia R.. et al. Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines. CANCER METAB. 2022 Dec;10(1):1-16} {FCM} {Human}
[IF={{ 4.35 }}] {Chuang, Yao-Chung, et al. "Peroxisome proliferator activated receptors γ/mitochondrial uncoupling protein 2 signaling protects against seizure-induced neuronal cell death in the hippocampus following experimental status} {Other} {="Rat"}
[IF={{ 4.35 }}] {Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227} {WB} {Mouse}
[IF={{ 2.6 }}] {Yaning Biao. et al. Wulingsan Alleviates MAFLD by Activating Autophagy via Regulating the AMPK/mTOR/ULK1 Signaling Pathway. CAN J GASTROENTEROL. 2024 Jul;2024(1):9777866} {IHC} {Rat}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Phenotypic Analysis of BAT versus WAT Differentiation
BAT for UCP1 protein. Left panels show the DAPI nuclear counterstaining; right panels show the Alexa647 signal of the conjugated secondary antibody. Top: IgG isotype control as primary antibody; bottom: rabbit anti UCP1 primary antibody
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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