Cytokeratin 5 Rabbit pAb(bs-1060R)-50ul/100ul/200ul产品图
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Cytokeratin 5 Rabbit pAb(bs-10

60R)-50ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-1060R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号 bs-1060R
    英文名称 Cytokeratin 5 Rabbit pAb
    中文名称 细胞角蛋白5抗体
    英文别名 Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; CK 5; CK5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; DDD; EBS 2; EBS2; K5; Keratin 5(epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5; Cytokeratin-5; Cytokeratin5; DDD; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; Keratin 5; Keratin; Keratin Type II Cytoskeletal 5; Keratin5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5.
    产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.
    交叉反应 Human, Mouse, Rat (Dog, Pig, Cow, Horse, Rabbit)
    抗体来源 Rabbit
    免疫原 KLH conjugated synthetic peptide derived from human CK5
    亚型 IgG
    性状 Liquid
    纯化方法 affinity purified by Protein A
    克隆类型 Polyclonal
    理论分子量 64 kDa
    浓度 1mg/ml
    储存液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class I > Cytokeratins
    亚基 Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
    相似性 Belongs to the intermediate filament family.
    保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料 Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.

     

    应用 推荐稀释比例
    {WB} {1:500-2000}
    {IHC-P} {1:100-500}
    {IHC-F} {1:100-500}
    {IF} {1:100-500}

     

    P‌‌araformaldehyde-fixed, paraffin embedded (rat bladder tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (bs-1060R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    P‌‌araformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (bs-1060R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片1
    Sample:
    Lane 1: Skin (Mouse) Lysate at 40 ug
    Lane 2: Uterus (Mouse) Lysate at 40 ug
    Lane 3: Urinary bladder (Mouse) Lysate at 40 ug
    Lane 4: Esophagus (Mouse) Lysate at 40 ug
    Lane 5: Skin (Rat) Lysate at 40 ug
    Lane 6: Uterus (Rat) Lysate at 40 ug
    Lane 7: Urinary bladder (Rat) Lysate at 40 ug
    Lane 8: Esophagus (Rat) Lysate at 40 ug
    Primary: Anti-Cytokeratin 5 (bs-1060R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 62 kD
    Observed band size: 60 kD
    P‌‌araformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (bs-1060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    文献支持
    Cytokeratin 5 Rabbit pAb(bs-1060R)-50ul/100ul/200ul
    ￥1180 - 2800