Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-C5a (bs-0324R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115/105 kD
Observed band size: 115 kD
- ￥1180 - 2800
- Bioss
- bs-0324R
- 2025年10月24日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0324R |
| 英文名称 | C5a anaphylatoxin Rabbit pAb |
| 中文名称 | 末端补体复合物抗体 |
| 英文别名 | C5; C6; C7; C8; C9; Complement component 5; Complement component 6; Complement component 7; Complement component 8; Complement component 9; MAC; Membrane attack complex; TCC; Terminal complement complex. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | Purified Human SC5b-9 Complex |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 8/109 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Immunology > Innate Immunity > Complement > Alternative Pathway Immunology > Innate Immunity > Complement > Classical Pathway Immunology > Innate Immunity > Complement > MAC Immunology > Innate Immunity > Macrophage / Inflamm. Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚基 | C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Interacts with tick complement inhibitor. |
| 亚细胞定位 | Secreted. |
| 相似性 | Contains 1 anaphylatoxin-like domain. Contains 1 NTR domain. |
| 功能 | Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2ug/Test} |
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文献和实验该产品被引用文献
[IF={{ 6.1 }}] {Ayako Kashimada. et al. De novo variant in RING finger protein 213 causes systemic vasculopathy. JCI INSIGHT. 2025 Jun;10(11):190094} {IHC} {Mouse}
[IF={{ 0 }}] {Miyabe, Yoshishige, et al. "Complement C5a receptor is the key initiator of neutrophil adhesion igniting immune complex–induced arthritis." Science Immunology 2.7 (2017): eaaj2195.} {IHC} {="Mouse"}
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C5a anaphylatoxin Rabbit pAb(bs-0324R)-50ul/100ul/200ul
￥1180 - 2800
