Sample:
_x000D_
Jurkat(Human) Cell Lysate at 30 ug
_x000D_
Primary: Anti-Fas Ligand (bs-0216R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 31 kD
_x000D_
Observed band size: 40 kD
- ￥1180 - 2800
- Bioss
- bs-0216R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0216R |
| 英文名称 | Fas Ligand Rabbit pAb |
| 中文名称 | FasL（CD178）抗体 |
| 英文别名 | Tumor necrosis factor ligand superfamily member 6; FASLG; APO1L; Apoptosis(APO 1) antigen ligand 1; Fas-L; Apoptosis antigen ligand 1; APTL; CD178; CD-178; CD 178; CD95L; CD95 ligand; CD95-L; CD95L protein; FAS antigen ligand; Fas L; FASL; Fas ligand(TNF superfamily member 6); Generalized lymphoproliferative disease; TNF superfamily member 6; TNFSF6; TNFL6_HUMAN; Apoptosis antigen ligand; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; FASLG. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Fas Ligand |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 31 kDa |
| 实际分子量 | 38-42 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. |
| 亚细胞定位 | Cell membrane; Single-pass type II membrane protein. Secreted. Cytoplasmic vesicle lumen. Lysosome lumen. Note=May be released into the extracellular fluid, probably by cleavage form the cell surface. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. |
| 翻译后修饰 | N-glycosylated._x000D_
The soluble form derives from the membrane form by proteolytic processing._x000D_ Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization._x000D_ Monoubiquitinated. |
| 相似性 | Belongs to the tumor necrosis factor family. |
| 功能 | Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(bs-0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(bs-0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(bs-0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(bs-0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Tissue/cell: human rectal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(bs-0216R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(bs-0216R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_
Blank control: mouse thymouses(blue)
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), _x000D_ Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green)._x000D_
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), _x000D_ Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green)._x000D_
Blank control: Mouse Kidney (blue).
Primary Antibody:Rabbit Anti-phospho-Fas Ligand antibody (bs-0216R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0216R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20,000 events was performed._x000D_
Primary Antibody:Rabbit Anti-phospho-Fas Ligand antibody (bs-0216R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0216R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20,000 events was performed._x000D_
Sample: Bone (mouse) Lysate at 40 ug
_x000D_ Primary: Anti- Fas ligand (bs-0216R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 31 kD
_x000D_ Observed band size: 46 kD
_x000D_
_x000D_ Primary: Anti- Fas ligand (bs-0216R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 31 kD
_x000D_ Observed band size: 46 kD
_x000D_
Sample:
_x000D_ Lane 1: Bone (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Bone (Rat) Lysate at 40 ug
_x000D_ Primary: Anti-Fas Ligand (bs-0216R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 40 kD
_x000D_ Observed band size: 40 kD
_x000D_ Lane 1: Bone (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Bone (Rat) Lysate at 40 ug
_x000D_ Primary: Anti-Fas Ligand (bs-0216R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 40 kD
_x000D_ Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fas Ligand) Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_
_x000D_
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