Tissue/cell:bs-0036R human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated(bs-0036R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
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- bs-0036R
- 2026年10月13日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0036R |
| 英文名称 | phospho-CREB-1 (Ser133) Rabbit pAb |
| 中文名称 | 磷酸化CREB-1抗体 |
| 英文别名 | CREB(phospho-Ser133); p-CREB-1(Ser133); CREB(phospho S133); pCREB-1(Ser133); p-CREB(phospho S133); Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser133 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 37 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH / Leucine Zipper Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Immunology > Innate Immunity > TLR Signaling |
| 亚基 | Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity (By similarity). Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. _x000D_
Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. |
| 相似性 | Belongs to the bZIP family._x000D_
Contains 1 bZIP domain._x000D_ Contains 1 KID (kinase-inducible) domain. |
| 功能 | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF-2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated(bs-0036R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated(bs-0036R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_
Blank control: RSC96(blue), the cells were fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), _x000D_ Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green)._x000D_
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), _x000D_ Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green)._x000D_
P-CREB1 was immunoprecipitated from mouse kidney tissue with bs-0036R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
_x000D_ Lane 1: mouse kidney tissue lysate 10 µg (Input).
_x000D_ Lane 2: bs-0036R IP in mouse kidney tissue lysate.
_x000D_ Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control).
_x000D_ Secondary
_x000D_ All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
_x000D_
_x000D_ Lane 1: mouse kidney tissue lysate 10 µg (Input).
_x000D_ Lane 2: bs-0036R IP in mouse kidney tissue lysate.
_x000D_ Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control).
_x000D_ Secondary
_x000D_ All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
_x000D_
Sample:
_x000D_ Lane 1: Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Cerebellum (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Kidney (Mouse) Lysate at 40 ug
_x000D_ Lane 4: Huvec (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-phospho-CREB-1 (Ser133) (bs-0036R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 43 kD
_x000D_ Observed band size: 45 kD
_x000D_ Lane 1: Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Cerebellum (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Kidney (Mouse) Lysate at 40 ug
_x000D_ Lane 4: Huvec (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-phospho-CREB-1 (Ser133) (bs-0036R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 43 kD
_x000D_ Observed band size: 45 kD
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CREB1 (p-S133) Rabbit pAb(bs-0036R)-50ul/100ul/200ul
￥1180 - 2800
