[ PROPERTIES ]
Residues: Met1~Pro302
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q96M86
Host: E. coli
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.5
Predicted Molecular Mass: 64.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MVPEERRVGL SSDETSSDSL KSWHSICVLD SKEQPLACQQ KQRQFVKPVT ESEQPTVLEL
LLAELRTLFS AVLQDSSPAA WRYLHAVLGL LPPYRELLVG HLDLLPFLEQ LYCWAPWVQT
HLHLDLLGAI VQAFPPDSSL LDSASHADCC PQKRRLHHRP PCPACPFVQA QWSRQQVKEE
LATWLRPLTL PELQRCLGIV GAQVALEEAV WLDGLSLLPL ALAADIPVRY ESSDTDNAEV
EPVGRKETRS QLDYEVPREK AFQKSSTGFS PETSFLDSQV MTALKMERYL KKIHFLYLNV
AP