Sample:
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 43 kD
- ￥1180 - 5600
- Bioss
- bsm-33190M
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul/200ug
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-33190M |
| 英文名称 | Caspase 8 Mouse mAb |
| 中文名称 | 半胱氨酸蛋白酶8单克隆抗体 |
| 英文别名 | Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; Caspase-8; Caspase8; CASP8; CASP8_HUMAN; Caspase8; Caspase 8 apoptosis related cysteine peptidase; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH antibody MACH beta 1/2/3/4 protein antibody MCH 5; MCH5. |
| 产品应用 | WB=1:2000-5000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human Caspase 8 Protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 43/57 kDa |
| 实际分子量 | 43kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Apoptosis > Apoptosis Markers > Caspases Cancer > Cell Death > Apoptosis > Metabolism Cancer > Cell Death > Necroptosis Cancer > Invasion/microenvironment > Apoptosis > Caspases Cell Biology > Apoptosis > Intracellular > Caspases etc > Caspases Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases Metabolism > Pathways and Processes > Metabolism processes > Apoptosis |
| 亚基 | Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. |
| 相似性 | Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. |
| 功能 | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:2000-5000} |
Sample:
Stomach(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Urinary bladder(Mouse) Lysate at 40 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 55'53D
Stomach(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Urinary bladder(Mouse) Lysate at 40 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 55'53D
Sample:
293T-UV(Human) Cell Lysate at 30 ug
U251-UV(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41’43’55 kD
Observed band size: 43 kD
293T-UV(Human) Cell Lysate at 30 ug
U251-UV(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41’43’55 kD
Observed band size: 43 kD
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Caspase 8 Mouse mAb(bsm-33190M)-50ul/100ul/200ul/200ug
￥1180 - 5600
