Sample: Liver(mouse) Lysate at 40 ug
_x000D_
Primary: Anti-eNOS (bsm-33176M)at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
_x000D_
Predicted band size: 133kD
_x000D_
Observed band size: 135kD
_x000D_
- ￥1180 - 5600
- Bioss
- bsm-33176M
- 2026年10月13日
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50ul/100ul/200ul/200ug
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-33176M |
| 英文名称 | eNOS Mouse mAb |
| 中文名称 | 一氧化氮合成酶-3（内皮型）单克隆抗体 |
| 英文别名 | NOS-3; cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3(endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN. |
| 产品应用 | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human eNos |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 133 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cardiovascular > Blood > Platelets Cardiovascular > Vasculature > Vasodilation > Nitric oxide associated Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Neuroscience > Neurotransmission > Nitric Oxide > NOS |
| 亚基 | Homodimer. Interacts with NOSIP and NOSTRIN. |
| 亚细胞定位 | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| 组织特异性 | Platelets, placenta, liver and kidney. |
| 翻译后修饰 | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. |
| 相似性 | Belongs to the NOS family. _x000D_ Contains 1 FAD-binding FR-type domain. _x000D_ Contains 1 flavodoxin-like domain. |
| 功能 | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets._x000D_
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Cerebrum (Rat) Lysate at 40 ug
_x000D_ Lung (Mouse) Lysate at 40 ug
_x000D_ Placenta (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-eNOS (bsm-33176M) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
_x000D_ Predicted band size: 135 kD
_x000D_ Observed band size: 135 kD
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Cerebrum (Rat) Lysate at 40 ug
_x000D_ Lung (Mouse) Lysate at 40 ug
_x000D_ Placenta (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-eNOS (bsm-33176M) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
_x000D_ Predicted band size: 135 kD
_x000D_ Observed band size: 135 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (eNOS/NOS-3) Monoclonal Antibody, Unconjugated (bsm-33176M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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文献和实验相关实验
-dependent and constitutively expressed and produce low levels of NO: NOS1 (neuronal NOS or nNOS), which is found mostly in neurons and skeletal muscle, and NOS3 (endothelial NOS or eNOS), which is found mostly in endothelial cells. NOS1 is critical
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NOS3 | eNOS Mouse mAb(bsm-33176M)-50ul/100ul/200ul/200ug
￥1180 - 5600
