HP1 alpha Mouse mAb(bsm-33377M)-50ul/100ul/200ul/200ug
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HP1 alpha Mouse mAb(bsm-33377M

)-50ul/100ul/200ul/200ug
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  • ￥1180 - 5600
  • Bioss已认证
  • bsm-33377M
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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    • 规格：

      50ul/100ul/200ul/200ug

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    规格：200ug产品价格：￥5600.0
    产品编号 bsm-33377M
    英文名称 HP1 alpha Mouse mAb
    中文名称 异染色质蛋白1-α单克隆抗体
    英文别名 Antigen p25; HP1-α; CBX5; CBX5_HUMAN; Chromobox homolog 5(HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha.
    中文别名 HP-1-α; HP 1α; HP-1α; HP-1 α; HP1α; HP1 α;
    产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.
    交叉反应 Human, 肾, 肾, 肾, 肾, Prostate Tumor, Prostate Tumor, Prostate Tumor, Prostate Tumor, Human Glioma, Human Glioma, Human Glioma, Human Glioma, human cervical carcinoma, human cervical carcinoma, human cervical carcinoma, human cervical carcinoma, 肾, 肾, 肾, Prostate Tumor, Prostate Tumor, Prostate Tumor, Human Glioma, Human Glioma, Human Glioma, human cervical carcinoma, human cervical carcinoma, human cervical carcinoma (Mouse, Rat)
    抗体来源 Mouse
    免疫原 Recombinant human HP1 alpha Protein
    亚型 IgG
    性状 Liquid
    纯化方法 affinity purified by Protein G
    克隆类型 Monoclonal
    理论分子量 22 kDa
    浓度 1mg/ml
    储存液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > Methyl Lysine

    Tags & Cell Markers > Subcellular Markers > Nucleus > Chromatin
    亚基

    Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain (PubMed:15882967, PubMed:9169472).
    Can interact directly with CBX3 via the chromoshadow domain (PubMed:9169472).
    Interacts with KMT5B and KMT5C (By similarity).
    Interacts with HP1BP3 (PubMed:20042602).
    Interacts with histone H3 methylated at 'Lys-9' (PubMed:11242053, PubMed:21047797).
    Interacts with BAHD1, MIS12 and DSN1 (PubMed:15502821, PubMed:19666599).
    Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5 (PubMed:20562864, PubMed:20850016, PubMed:21346195).
    Interacts with INCENP and TRIM24 (PubMed:11313457).
    Interacts with CHAMP1 (PubMed:20850016).
    Interacts with PRR14 (via N-terminus) (PubMed:25906157).
    Interacts with RRP1B (PubMed:19710015).
    Interacts with HNRNPU (via C-terminus); this interaction is, at least in part, RNA-dependent (PubMed:19617346).
    Interacts with LRIF1 (via PxVxL motif) (PubMed:23542155).
    Interacts with ZNF263; recruited to the SIX3 promoter along with other proteins involved in chromatin modification and transcriptional corepression where it contributes to transcriptional repression (PubMed:32051553).
    May form homodimers (By similarity).
    Interacts with SMYD5 (By similarity).
    Interacts with CHD3 (PubMed:28977666).
    Interacts with CHD4 (PubMed:28977666).
    亚细胞定位

    Nucleus. Chromosome. Chromosome, centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
    翻译后修饰

    Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity).
    Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis
    相似性

    Contains 2 chromo domains.
    功能

    Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
    保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料 This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq].

     

    应用 推荐稀释比例
    {WB} {1:500-2000}
    {IHC-P} {1:100-500}
    {IHC-F} {1:100-500}
    {IF} {1:100-500}

     

    HP1 alpha Mouse mAb(bsm-33377M
    Sample:
    MOLT-4(Human) Cell Lysate at 30 ug
    Siha(Human) Cell Lysate at 30 ug
    A549(Human) Cell Lysate at 30 ug
    Primary: Anti- HP1 alpha (bsm-33377M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
    Predicted band size: 22 kD
    Observed band size: 22 kD
    P‌‌araformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    P‌‌araformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    P‌‌araformaldehyde-fixed, paraffin embedded (human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    P‌‌araformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 7.666 }}] {Sarah C. Wistner. et al. Characterization of Hepatoma-Derived Growth Factor-Related Protein 2 Interactions with Heterochromatin. CELLS-BASEL. 2023 Jan;12(2):325} {WB} {Human}

    [IF={{ 3.3 }}] {Chuanling Wang. et al. Histone MARylation regulates lipid metabolism in colorectal cancer by promoting IGFBP1 methylation. EXP CELL RES. 2024 Oct;:114308} {IF} {Human}

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    文献支持
    HP1 alpha Mouse mAb(bsm-33377M)-50ul/100ul/200ul/200ug
    ￥1180 - 5600