Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-CT054 antibody (bs-4164R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-4164R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4164R |
| 英文名称 | CT054 Rabbit pAb |
| 中文名称 | 核黄素转运蛋白2抗体 |
| 英文别名 | bA371L19.1; BVVLS; C20orf54; C20orf54provided by HGNC; Chromosome 20 open reading frame 54; hRFT2; MGC10698; S52A3_HUMAN; RFT2; Riboflavin transporter 2. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CT054 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 51 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Vitamins / minerals Signal Transduction > Metabolism > Vitamins / Minerals |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Predominantly expressed in testis. Highly expressed in small intestine and prostate. |
| 相似性 | Belongs to the riboflavin transporter family. |
| 功能 | Riboflavin transporter. Riboflavin transport is Na(+)-independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and to a lesser extent by amiloride. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CT054 is a Riboflavin transporter. Riboflavin transport is Na+-independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and to a lesser extent by amiloride. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {3ug/Test} |
Sample:
Testis (Rat) Lysate at 40 ug
Primary: Anti-CT054 (bs-4164R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Testis (Rat) Lysate at 40 ug
Primary: Anti-CT054 (bs-4164R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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文献和实验该产品被引用文献
[IF={{ 3.454 }}] {Anandam KY et al. Effect of the pro-inflammatory cytokine TNF-α on intestinal riboflavin uptake: Inhibition mediated via transcriptional mechanism(s). (2018) American Journal of Physiology-Cell Physiology.} {WB} {Mouse}
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CT054 Rabbit pAb(bs-4164R)-50ul/100ul/200ul
￥1180 - 2800
